{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kojima Y"],"funding":["JSPS KAKENHI","AMED","Japan Agency for Medical Research and Development","MEXT KAKENHI"],"pagination":["3054-3066"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11462933"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["115(9)"],"pubmed_abstract":["Austocystin D is a natural compound that induces cytochrome P450 (CYP) monooxygenase-dependent DNA damage and growth inhibition in certain cancer cell lines. Cancer cells exhibiting higher sensitivity to austocystin D often display elevated CYP2J2 expression. However, the essentiality and the role of CYP2J2 for the cytotoxicity of this compound remain unclear. In this study, we demonstrate that CYP2J2 depletion alleviates austocystin D sensitivity and DNA damage induction, while CYP2J2 overexpression enhances them. Moreover, the investigation into genes involved in austocystin D cytotoxicity identified POR and PGRMC1, positive regulators for CYP activity, and KAT7, a histone acetyltransferase. Through genetic manipulation and analysis of multiomics data, we elucidated a role for KAT7 in CY"],"journal":["Cancer science"],"pubmed_title":["Cytochrome P450 2J2 is required for the natural compound austocystin D to elicit cancer cell toxicity."],"pmcid":["PMC11462933"],"funding_grant_id":["JP19H05655","JP20K07037","JP20cm0106113"],"pubmed_authors":["Takikawa M","Zhou L","Kagaya N","Kawahara T","Kuramochi K","Ishikawa F","Dan S","Furuya T","Mizumoto A","Shin-Ya K","Kojima Y","Fujieda S","Tomida A","Sadaie M"],"additional_accession":[]},"is_claimable":false,"name":"Cytochrome P450 2J2 is required for the natural compound austocystin D to elicit cancer cell toxicity.","description":"Austocystin D is a natural compound that induces cytochrome P450 (CYP) monooxygenase-dependent DNA damage and growth inhibition in certain cancer cell lines. Cancer cells exhibiting higher sensitivity to austocystin D often display elevated CYP2J2 expression. However, the essentiality and the role of CYP2J2 for the cytotoxicity of this compound remain unclear. In this study, we demonstrate that CYP2J2 depletion alleviates austocystin D sensitivity and DNA damage induction, while CYP2J2 overexpression enhances them. Moreover, the investigation into genes involved in austocystin D cytotoxicity identified POR and PGRMC1, positive regulators for CYP activity, and KAT7, a histone acetyltransferase. Through genetic manipulation and analysis of multiomics data, we elucidated a role for KAT7 in CY","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Sep","modification":"2025-04-26T20:25:47.799Z","creation":"2025-04-06T16:25:16.783Z"},"accession":"S-EPMC11462933","cross_references":{"pubmed":["39009033"],"doi":["10.1111/cas.16289"]}}