biostudies-literatureUnknownHundley FVNINDS NIH HHSNIGMS NIH HHSPlasma membrane protein degradation and recycling is regulated by the endolysosomal system, wherein endosomes bud from the plasma membrane into the cytosol and mature into degradative lysosomes. As such, the endolysosomal system plays a critical role in determining the abundance of proteins on the cell surface, influencing cellular identity and function. Highly polarized cells, like neurons, rely on the endolysosomal system for axonal and dendritic specialization and synaptic compartmentalization. The importance of this system to neuronal function is reflected by the prevalence of risk variants in components of the system in several neurodegenerative diseases, ranging from Parkinson's to Alzheimer's disease. Nevertheless, our understanding of endocytic cargo and core endolysosomal machinerbioRxiv : the preprint server for biology2024.09.24.614704https://www.ebi.ac.uk/biostudies/studies/S-EPMC11463543biostudies-literatureEndo-IP and Lyso-IP Toolkit for Endolysosomal Profiling of Human Induced Neurons.PMC11463543R01 GM132129R01 NS083524R01 NS110395Harper JWHundley FVZhang JPaulo JAGottschalk LMCook ANKGonzalez-Lozano MAfalseEndo-IP and Lyso-IP Toolkit for Endolysosomal Profiling of Human Induced Neurons.Plasma membrane protein degradation and recycling is regulated by the endolysosomal system, wherein endosomes bud from the plasma membrane into the cytosol and mature into degradative lysosomes. As such, the endolysosomal system plays a critical role in determining the abundance of proteins on the cell surface, influencing cellular identity and function. Highly polarized cells, like neurons, rely on the endolysosomal system for axonal and dendritic specialization and synaptic compartmentalization. The importance of this system to neuronal function is reflected by the prevalence of risk variants in components of the system in several neurodegenerative diseases, ranging from Parkinson's to Alzheimer's disease. Nevertheless, our understanding of endocytic cargo and core endolysosomal machiner2024-01-01T00:00:00Z2024 Sep2026-04-08T19:00:35.057Z2025-04-04T09:58:44.892ZS-EPMC114635433938650210.1101/2024.09.24.614704