{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wang Y"],"funding":["Fundamental Research Funds for the Central Universities","National Key R&D Program of China","National Key R&D Program of China","Key research and development project of Hubei Province","National Nature Science Foundation of China"],"pagination":["RP98949"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11464005"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13"],"pubmed_abstract":["Acute retinal ischemia and ischemia-reperfusion injury are the primary causes of retinal neural cell death and vision loss in retinal artery occlusion (RAO). The absence of an accurate mouse model for simulating the retinal ischemic process has hindered progress in developing neuroprotective agents for RAO. We developed a unilateral pterygopalatine ophthalmic artery occlusion (UPOAO) mouse model using silicone wire embolization combined with carotid artery ligation. The survival of retinal ganglion cells and visual function were evaluated to determine the duration of ischemia. Immunofluorescence staining, optical coherence tomography, and haematoxylin and eosin staining were utilized to assess changes in major neural cell classes and retinal structure degeneration at two reperfusion durati"],"journal":["eLife"],"pubmed_title":["Transcriptional responses in a mouse model of silicone wire embolization induced acute retinal artery ischemia and reperfusion."],"pmcid":["PMC11464005"],"funding_grant_id":["2042023gf0013","82371079","2023YFC2308404","2022BCA009"],"pubmed_authors":["Li Y","Li Z","Feng J","Xie H","Wang C","Lv B","Xiao X","Wang F","Wan Y","Yang A","Wang Y","Chen T"],"additional_accession":[]},"is_claimable":false,"name":"Transcriptional responses in a mouse model of silicone wire embolization induced acute retinal artery ischemia and reperfusion.","description":"Acute retinal ischemia and ischemia-reperfusion injury are the primary causes of retinal neural cell death and vision loss in retinal artery occlusion (RAO). The absence of an accurate mouse model for simulating the retinal ischemic process has hindered progress in developing neuroprotective agents for RAO. We developed a unilateral pterygopalatine ophthalmic artery occlusion (UPOAO) mouse model using silicone wire embolization combined with carotid artery ligation. The survival of retinal ganglion cells and visual function were evaluated to determine the duration of ischemia. Immunofluorescence staining, optical coherence tomography, and haematoxylin and eosin staining were utilized to assess changes in major neural cell classes and retinal structure degeneration at two reperfusion durati","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Oct","modification":"2026-07-14T19:41:50.678Z","creation":"2025-04-04T01:54:35.68Z"},"accession":"S-EPMC11464005","cross_references":{"pubmed":["39382568"],"doi":["10.7554/eLife.98949"]}}