biostudies-literatureUnknown13(11)Li ZGlucose-like peptide-1 (GLP-1) is a vital hormone in the intestines that regulates glucose metabolism. Although pancreatic-derived factor (PANDER) overexpression is known to suppress GLP-1, the underlying mechanisms are unclear. Our study aims to uncover how PANDER influences GLP-1 synthesis and secretion. We established a PANDER overexpression model in STC-1 intestinal cells, confirming its inhibitory effect on GLP-1 secretion. This effect was reversed in PANDER-knockout cells. Additionally, a negative correlation between PANDER and GLP-1 was observed in patients with a history of gestational diabetes. Subsequently, through whole transcriptome gene sequencing in PANDER-overexpressed STC-1 cells, we discovered that the activation of IL-6 and its related STAT3 signaling pathway was significEndocrine connectionse230548https://www.ebi.ac.uk/biostudies/studies/S-EPMC11466252biostudies-literatureThe role of PANDER and its interplay with IL-6 in the regulation of GLP-1 secretion.PMC11466252Yue SChen NLi YLi ZCao XPei LLai FXiao HXu CfalseThe role of PANDER and its interplay with IL-6 in the regulation of GLP-1 secretion.Glucose-like peptide-1 (GLP-1) is a vital hormone in the intestines that regulates glucose metabolism. Although pancreatic-derived factor (PANDER) overexpression is known to suppress GLP-1, the underlying mechanisms are unclear. Our study aims to uncover how PANDER influences GLP-1 synthesis and secretion. We established a PANDER overexpression model in STC-1 intestinal cells, confirming its inhibitory effect on GLP-1 secretion. This effect was reversed in PANDER-knockout cells. Additionally, a negative correlation between PANDER and GLP-1 was observed in patients with a history of gestational diabetes. Subsequently, through whole transcriptome gene sequencing in PANDER-overexpressed STC-1 cells, we discovered that the activation of IL-6 and its related STAT3 signaling pathway was signific2024-01-01T00:00:00Z2024 Oct2026-06-07T07:41:40.967Z2025-04-04T11:05:47.257ZS-EPMC114662523923585910.1530/EC-23-0548