biostudies-literatureLiu Jthe Foundation of the committee on science and technology of Tianjinthe Joint Scientific Research Project between Nankai University and Tianjin Union Medical CenterTianjin Key Medical Discipline (Specialty) Construction Project23661https://www.ebi.ac.uk/biostudies/studies/S-EPMC11467296biostudies-literatureUnknown14(1)Etoposide is a second-line chemotherapy agent widely used for metastatic colorectal cancer. However, we discovered that etoposide treatment induced greater motility potential in four colorectal cancer cell lines. Therefore, we used microarrays to test the mRNA of these cancer cell lines to investigate the mechanisms of etoposide promoting colorectal cancer metastasis. Differentially expressed genes (DEGs) were identified by comparing the gene expression profiles in samples from etoposide-treated cells and untreated cells in all four colorectal cancer cell lines. Next, these genes went through the Gene Set Enrichment Analysis (GSEA), Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) Pathway analysis. Among the top 10 genes including the upregulated and downregulated,Scientific reportsMicroarray analysis points to LMNB1 and JUN as potential target genes for predicting metastasis promotion by etoposide in colorectal cancer.PMC11467296TJYXZDXK-044A20JCQNJC018702016rmnk002Yang HLiu JZeng QZhang ZZhang XLi PZhou YSun YfalseMicroarray analysis points to LMNB1 and JUN as potential target genes for predicting metastasis promotion by etoposide in colorectal cancer.Etoposide is a second-line chemotherapy agent widely used for metastatic colorectal cancer. However, we discovered that etoposide treatment induced greater motility potential in four colorectal cancer cell lines. Therefore, we used microarrays to test the mRNA of these cancer cell lines to investigate the mechanisms of etoposide promoting colorectal cancer metastasis. Differentially expressed genes (DEGs) were identified by comparing the gene expression profiles in samples from etoposide-treated cells and untreated cells in all four colorectal cancer cell lines. Next, these genes went through the Gene Set Enrichment Analysis (GSEA), Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) Pathway analysis. Among the top 10 genes including the upregulated and downregulated,2024-01-01T00:00:00Z2024 Oct2025-04-22T10:56:22.616Z2025-04-05T23:45:43.258ZS-EPMC114672963939000210.1038/s41598-024-72674-8