{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Huang W"],"funding":["NIDDK NIH HHS","NCI NIH HHS","NIDDK","National Cancer Institute Division of Cancer Prevention"],"pagination":["110930"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11467673"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["27(10)"],"pubmed_abstract":["AAV vectors are mainstream delivery platforms in gene therapy, yet AAV-mediated gene transfer to adipose tissue is underdeveloped due to low efficiency of natural AAVs. We previously demonstrated that an engineered capsid Rec2 displayed improved adipo-tropism but with the caveat of liver transduction. To generate highly adipo-tropic capsid, we modified Rec2 capsid by site-specific mutagenesis and found the variant V7 with F503Y, Y708D and K709I substitution to harbor highly selective adipo-tropism while diminishing liver transduction. Intraperitoneal injection favored transduction to visceral fat while intravenous administration favored subcutaneous fat. Intraperitoneal administration of V7 vector harboring human leptin and adiponectin as single transcript normalized the metabolic dysfunct"],"journal":["iScience"],"pubmed_title":["Development of an adipose-tropic AAV capsid ablating liver tropism."],"pmcid":["PMC11467673"],"funding_grant_id":["R01 CA166590","R01 DK137431"],"pubmed_authors":["Cao L","Huang W","Bates R","Mohammed T","Appana B"],"additional_accession":[]},"is_claimable":false,"name":"Development of an adipose-tropic AAV capsid ablating liver tropism.","description":"AAV vectors are mainstream delivery platforms in gene therapy, yet AAV-mediated gene transfer to adipose tissue is underdeveloped due to low efficiency of natural AAVs. We previously demonstrated that an engineered capsid Rec2 displayed improved adipo-tropism but with the caveat of liver transduction. To generate highly adipo-tropic capsid, we modified Rec2 capsid by site-specific mutagenesis and found the variant V7 with F503Y, Y708D and K709I substitution to harbor highly selective adipo-tropism while diminishing liver transduction. Intraperitoneal injection favored transduction to visceral fat while intravenous administration favored subcutaneous fat. Intraperitoneal administration of V7 vector harboring human leptin and adiponectin as single transcript normalized the metabolic dysfunct","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Oct","modification":"2026-07-15T13:06:55.712Z","creation":"2025-04-04T07:40:42.52Z"},"accession":"S-EPMC11467673","cross_references":{"pubmed":["39398244"],"doi":["10.1016/j.isci.2024.110930"]}}