biostudies-literatureLuken ADeutsche ForschungsgemeinschaftHorizon 2020 Framework Programmee2100898https://www.ebi.ac.uk/biostudies/studies/S-EPMC11468244biostudies-literatureUnknown10(20)For successful material deployment in tissue engineering, the material itself, its mechanical properties, and the microscopic geometry of the product are of particular interest. While silk is a widely applied protein-based tissue engineering material with strong mechanical properties, the size and shape of artificially spun silk fibers are limited by existing processes. This study adjusts a microfluidic spinneret to manufacture micron-sized wet-spun fibers with three different materials enabling diverse geometries for tissue engineering applications. The spinneret is direct laser written (DLW) inside a microfluidic polydimethylsiloxane (PDMS) chip using two-photon lithography, applying a novel surface treatment that enables a tight print-channel sealing. Alginate, polyacrylonitrile, and siAdvanced healthcare materialsBiocompatible Micron-Scale Silk Fibers Fabricated by Microfluidic Wet Spinning.PMC11468244WE 4678/12‐1“BioComb4Nanofibers” 862016436799631Joel ACLinkhorst JGeiger MLampert ASteinbeck LWessling MLuken AfalseBiocompatible Micron-Scale Silk Fibers Fabricated by Microfluidic Wet Spinning.For successful material deployment in tissue engineering, the material itself, its mechanical properties, and the microscopic geometry of the product are of particular interest. While silk is a widely applied protein-based tissue engineering material with strong mechanical properties, the size and shape of artificially spun silk fibers are limited by existing processes. This study adjusts a microfluidic spinneret to manufacture micron-sized wet-spun fibers with three different materials enabling diverse geometries for tissue engineering applications. The spinneret is direct laser written (DLW) inside a microfluidic polydimethylsiloxane (PDMS) chip using two-photon lithography, applying a novel surface treatment that enables a tight print-channel sealing. Alginate, polyacrylonitrile, and si2021-01-01T00:00:00Z2021 Oct2026-06-08T06:24:16.134Z2025-04-04T02:17:04.548ZS-EPMC114682443433152410.1002/adhm.202100898