{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Janzen D"],"funding":["Deutsche Forschungsgemeinschaft"],"pagination":["e2201826"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11468248"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["11(21)"],"pubmed_abstract":["3D neuronal cultures attempt to better replicate the in vivo environment to study neurological/neurodegenerative diseases compared to 2D models. A challenge to establish 3D neuron culture models is the low elastic modulus (30-500 Pa) of the native brain. Here, an ultra-soft matrix based on thiolated hyaluronic acid (HA-SH) reinforced with a microfiber frame is formulated and used. Hyaluronic acid represents an essential component of the brain extracellular matrix (ECM). Box-shaped frames with a microfiber spacing of 200 µm composed of 10-layers of poly(ɛ-caprolactone) (PCL) microfibers (9.7 ± 0.2 µm) made via melt electrowriting (MEW) are used to reinforce the HA-SH matrix which has an elastic modulus of 95 Pa. The neuronal viability is low in pure HA-SH matrix, however, when astrocytes ar"],"journal":["Advanced healthcare materials"],"pubmed_title":["Reinforced Hyaluronic Acid-Based Matrices Promote 3D Neuronal Network Formation."],"pmcid":["PMC11468248"],"funding_grant_id":["326 998 133 – TRR 225"],"pubmed_authors":["Villmann C","Budday S","Pal A","Andrade Mier M","Forster L","Detsch R","Faber J","Dalton PD","Bakirci E","Hauptstein J","Teßmar J","Blunk T","Hazur J","Boccaccini AR","Janzen D"],"additional_accession":[]},"is_claimable":false,"name":"Reinforced Hyaluronic Acid-Based Matrices Promote 3D Neuronal Network Formation.","description":"3D neuronal cultures attempt to better replicate the in vivo environment to study neurological/neurodegenerative diseases compared to 2D models. A challenge to establish 3D neuron culture models is the low elastic modulus (30-500 Pa) of the native brain. Here, an ultra-soft matrix based on thiolated hyaluronic acid (HA-SH) reinforced with a microfiber frame is formulated and used. Hyaluronic acid represents an essential component of the brain extracellular matrix (ECM). Box-shaped frames with a microfiber spacing of 200 µm composed of 10-layers of poly(ɛ-caprolactone) (PCL) microfibers (9.7 ± 0.2 µm) made via melt electrowriting (MEW) are used to reinforce the HA-SH matrix which has an elastic modulus of 95 Pa. The neuronal viability is low in pure HA-SH matrix, however, when astrocytes ar","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Nov","modification":"2025-04-26T07:56:41.74Z","creation":"2025-04-06T12:30:58.639Z"},"accession":"S-EPMC11468248","cross_references":{"pubmed":["35993391"],"doi":["10.1002/adhm.202201826"]}}