biostudies-literatureCosta JNYSão Paulo Research FoundationBrazilian National Council for Scientific and Technological DevelopmentBrazilian Coordination for Improvement of Higher Education PersonnelFundação de Amparo à Pesquisa do Estado de São Pauloe2303509https://www.ebi.ac.uk/biostudies/studies/S-EPMC11468374biostudies-literatureUnknown13(11)Multiplexing is a valuable strategy to boost throughput and improve clinical accuracy. Exploiting the vertical, meshed design of reproducible and low-cost ultra-dense electrochemical chips, the unprecedented single-response multiplexing of typical label-free biosensors is reported. Using a cheap, handheld one-channel workstation and a single redox probe, that is, ferro/ferricyanide, the recognition events taking place on two spatially resolved locations of the same working electrode can be tracked along a single voltammetry scan by collecting the electrochemical signatures of the probe in relation to different quasi-reference electrodes, Au (0 V) and Ag/AgCl ink (+0.2 V). This spatial isolation prevents crosstalk between the redox tags and interferences over functionalization and binding sAdvanced healthcare materialsSingle-Response Duplexing of Electrochemical Label-Free Biosensor from the Same Tag.PMC114683742022/04397-188887.663312/2022-00407951/2021-02022/04397‐12023/02080‐3304389/2019-62023/02080-3Lima RSAyres LBShimizu FMSanthiago MPaschoalino WJGarcia CDPiazzetta MHOVieira LCSPoker JAKubota LTCastro ACHMerces LCosta JNYPimentel GJCAlves WAGobbi ALfalseSingle-Response Duplexing of Electrochemical Label-Free Biosensor from the Same Tag.Multiplexing is a valuable strategy to boost throughput and improve clinical accuracy. Exploiting the vertical, meshed design of reproducible and low-cost ultra-dense electrochemical chips, the unprecedented single-response multiplexing of typical label-free biosensors is reported. Using a cheap, handheld one-channel workstation and a single redox probe, that is, ferro/ferricyanide, the recognition events taking place on two spatially resolved locations of the same working electrode can be tracked along a single voltammetry scan by collecting the electrochemical signatures of the probe in relation to different quasi-reference electrodes, Au (0 V) and Ag/AgCl ink (+0.2 V). This spatial isolation prevents crosstalk between the redox tags and interferences over functionalization and binding s2024-01-01T00:00:00Z2024 Apr2026-06-07T07:35:54.148Z2025-04-04T13:53:53.121ZS-EPMC114683743824583010.1002/adhm.202303509