biostudies-literatureStine JMNational Science Foundatione2302897https://www.ebi.ac.uk/biostudies/studies/S-EPMC11468942biostudies-literatureUnknown13(5)Hydrogen sulfide (H<sub>2</sub> S) is a gaseous inflammatory mediator and important signaling molecule for maintaining gastrointestinal (GI) homeostasis. Excess intraluminal H<sub>2</sub> S in the GI tract has been implicated in inflammatory bowel disease and neurodegenerative disorders; however, the role of H<sub>2</sub> S in disease pathogenesis and progression is unclear. Herein, an electrochemical gas-sensing ingestible capsule is developed to enable real-time, wireless amperometric measurement of H<sub>2</sub> S in GI conditions. A gold (Au) three-electrode sensor is modified with a Nafion solid-polymer electrolyte (Nafion-Au) to enhance selectivity toward H<sub>2</sub> S in humid environments. The Nafion-Au sensor-integrated capsule shows a linear current response in H<sub>2</sub> S Advanced healthcare materialsMiniaturized Capsule System Toward Real-Time Electrochemical Detection of H<sub>2</sub> S in the Gastrointestinal Tract.PMC114689421939236Levy JAGhodssi RBeardslee LAPasricha PJAbianeh HBotasini SStine JMRuland KLfalseMiniaturized Capsule System Toward Real-Time Electrochemical Detection of H<sub>2</sub> S in the Gastrointestinal Tract.Hydrogen sulfide (H<sub>2</sub> S) is a gaseous inflammatory mediator and important signaling molecule for maintaining gastrointestinal (GI) homeostasis. Excess intraluminal H<sub>2</sub> S in the GI tract has been implicated in inflammatory bowel disease and neurodegenerative disorders; however, the role of H<sub>2</sub> S in disease pathogenesis and progression is unclear. Herein, an electrochemical gas-sensing ingestible capsule is developed to enable real-time, wireless amperometric measurement of H<sub>2</sub> S in GI conditions. A gold (Au) three-electrode sensor is modified with a Nafion solid-polymer electrolyte (Nafion-Au) to enhance selectivity toward H<sub>2</sub> S in humid environments. The Nafion-Au sensor-integrated capsule shows a linear current response in H<sub>2</sub> S 2024-01-01T00:00:00Z2024 Feb2026-07-14T20:41:10.665Z2025-04-05T23:44:29.182ZS-EPMC114689423803572810.1002/adhm.202302897