{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ikeda Y"],"funding":["National Institute of Genetics","Japan Society for the Promotion of Science"],"pagination":["107735"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11470409"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["300(10)"],"pubmed_abstract":["VemP is a secretory protein in the Vibrio species that monitors cellular protein-transport activity through its translation arrest, allowing expression of the downstream secD2-secF2 genes in the same operon, which encode components of the protein translocation machinery. When cellular protein-transport function is fully active, secD2/F2 expression remains repressed as VemP translation arrest is canceled immediately. The VemP arrest cancellation occurs on the SecY/E/G translocon in a late stage in the translocation process and requires both trans factors, SecD/F and PpiD/YfgM, and a cis element, Arg-85 in VemP; however, the detailed molecular mechanism remains elusive. This study aimed to elucidate how VemP passing through SecY specifically monitors SecD/F function. Genetic and biochemical "],"journal":["The Journal of biological chemistry"],"pubmed_title":["Translation arrest cancellation of VemP, a secretion monitor in Vibrio, is regulated by multiple cis and trans factors, including SecY."],"pmcid":["PMC11470409"],"funding_grant_id":["23K05694","23K23835","23K23850","20K06556","22H02571"],"pubmed_authors":["Miyazaki R","Ikeda Y","Tsukazaki T","Akiyama Y","Mori H"],"additional_accession":[]},"is_claimable":false,"name":"Translation arrest cancellation of VemP, a secretion monitor in Vibrio, is regulated by multiple cis and trans factors, including SecY.","description":"VemP is a secretory protein in the Vibrio species that monitors cellular protein-transport activity through its translation arrest, allowing expression of the downstream secD2-secF2 genes in the same operon, which encode components of the protein translocation machinery. When cellular protein-transport function is fully active, secD2/F2 expression remains repressed as VemP translation arrest is canceled immediately. The VemP arrest cancellation occurs on the SecY/E/G translocon in a late stage in the translocation process and requires both trans factors, SecD/F and PpiD/YfgM, and a cis element, Arg-85 in VemP; however, the detailed molecular mechanism remains elusive. This study aimed to elucidate how VemP passing through SecY specifically monitors SecD/F function. Genetic and biochemical ","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Oct","modification":"2026-07-16T07:05:29.204Z","creation":"2025-04-04T01:17:08.606Z"},"accession":"S-EPMC11470409","cross_references":{"pubmed":["39233231"],"doi":["10.1016/j.jbc.2024.107735"]}}