{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["65(12)"],"submitter":["Gao ML"],"pubmed_abstract":["<h4>Purpose</h4>Microglia-like cells derived from stem cells (iMG) provide a plentiful cell source for studying the functions of microglia in both normal and pathological conditions. Our goal is to establish a simplified and effective method for generating iMG in a precisely defined system. Additionally, we aim to achieve functional maturation of iMG through coculture with retinal organoids.<h4>Methods</h4>In this study, iMG were produced under precisely defined conditions. They were subjected to LPS and poly IC stimulation. Additionally, we examined distinct phenotypic and functional variances between iMG and HMC3, a commonly used human microglia cell line. To investigate how the retinal cell interaction enhances microglial properties, iMG were cocultured with retinal organoids, producing"],"journal":["Investigative ophthalmology & visual science"],"pagination":["19"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11472886"],"repository":["biostudies-literature"],"pubmed_title":["Retinal Organoid Microenvironment Enhanced Bioactivities of Microglia-Like Cells Derived From HiPSCs."],"pmcid":["PMC11472886"],"pubmed_authors":["Wang TY","Guo Y","Zhang J","Cai JQ","Tang C","Lin X","Bai ZP","Chen LJ","Li M","Huang XF","Pan SH","Gao ML","Zeng SS","Liu ZC","Kong QR"],"additional_accession":[]},"is_claimable":false,"name":"Retinal Organoid Microenvironment Enhanced Bioactivities of Microglia-Like Cells Derived From HiPSCs.","description":"<h4>Purpose</h4>Microglia-like cells derived from stem cells (iMG) provide a plentiful cell source for studying the functions of microglia in both normal and pathological conditions. Our goal is to establish a simplified and effective method for generating iMG in a precisely defined system. Additionally, we aim to achieve functional maturation of iMG through coculture with retinal organoids.<h4>Methods</h4>In this study, iMG were produced under precisely defined conditions. They were subjected to LPS and poly IC stimulation. Additionally, we examined distinct phenotypic and functional variances between iMG and HMC3, a commonly used human microglia cell line. To investigate how the retinal cell interaction enhances microglial properties, iMG were cocultured with retinal organoids, producing","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Oct","modification":"2026-07-15T04:05:26.779Z","creation":"2025-04-04T01:24:43.807Z"},"accession":"S-EPMC11472886","cross_references":{"pubmed":["39392440"],"doi":["10.1167/iovs.65.12.19"]}}