biostudies-literatureUnknown65(12)Gao ML<h4>Purpose</h4>Microglia-like cells derived from stem cells (iMG) provide a plentiful cell source for studying the functions of microglia in both normal and pathological conditions. Our goal is to establish a simplified and effective method for generating iMG in a precisely defined system. Additionally, we aim to achieve functional maturation of iMG through coculture with retinal organoids.<h4>Methods</h4>In this study, iMG were produced under precisely defined conditions. They were subjected to LPS and poly IC stimulation. Additionally, we examined distinct phenotypic and functional variances between iMG and HMC3, a commonly used human microglia cell line. To investigate how the retinal cell interaction enhances microglial properties, iMG were cocultured with retinal organoids, producingInvestigative ophthalmology & visual science19https://www.ebi.ac.uk/biostudies/studies/S-EPMC11472886biostudies-literatureRetinal Organoid Microenvironment Enhanced Bioactivities of Microglia-Like Cells Derived From HiPSCs.PMC11472886Wang TYGuo YZhang JCai JQTang CLin XBai ZPChen LJLi MHuang XFPan SHGao MLZeng SSLiu ZCKong QRfalseRetinal Organoid Microenvironment Enhanced Bioactivities of Microglia-Like Cells Derived From HiPSCs.<h4>Purpose</h4>Microglia-like cells derived from stem cells (iMG) provide a plentiful cell source for studying the functions of microglia in both normal and pathological conditions. Our goal is to establish a simplified and effective method for generating iMG in a precisely defined system. Additionally, we aim to achieve functional maturation of iMG through coculture with retinal organoids.<h4>Methods</h4>In this study, iMG were produced under precisely defined conditions. They were subjected to LPS and poly IC stimulation. Additionally, we examined distinct phenotypic and functional variances between iMG and HMC3, a commonly used human microglia cell line. To investigate how the retinal cell interaction enhances microglial properties, iMG were cocultured with retinal organoids, producing2024-01-01T00:00:00Z2024 Oct2026-07-15T04:05:26.779Z2025-04-04T01:24:43.807ZS-EPMC114728863939244010.1167/iovs.65.12.19