{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Bansal A"],"funding":["Department of Biotechnology, Ministry of Science and Technology, India"],"pagination":["24076"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11473785"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14(1)"],"pubmed_abstract":["Conditional regulation is a highly beneficial system for studying the function of essential genes in Plasmodium falciparum and dimerizable Cre recombinase (DiCre) is a recently adapted conditional regulation system suitable for this purpose. In the DiCre system, two inactive fragments of Cre are reconstituted to form a functionally active enzyme in the presence of rapamycin. Different loci have been targeted to generate parasite lines that express the DiCre enzyme. Here, we have used marker-free CRISPR-Cas9 gene editing to integrate the DiCre cassette in a redundant cg6 locus. We have shown the utility of the newly generated ∆cg6DC4 parasites in mediating robust, rapid, and highly specific excision of exogenously encoded gfp sequence. The ∆cg6DC4 parasites are also capable of conditional e"],"journal":["Scientific reports"],"pubmed_title":["Generation of a new DiCre expressing parasite strain for functional characterization of Plasmodium falciparum genes in blood stages."],"pmcid":["PMC11473785"],"funding_grant_id":["BT/PR38411/GET/119/311/2020"],"pubmed_authors":["Bansal A","Choudhury H","Sharma M"],"additional_accession":[]},"is_claimable":false,"name":"Generation of a new DiCre expressing parasite strain for functional characterization of Plasmodium falciparum genes in blood stages.","description":"Conditional regulation is a highly beneficial system for studying the function of essential genes in Plasmodium falciparum and dimerizable Cre recombinase (DiCre) is a recently adapted conditional regulation system suitable for this purpose. In the DiCre system, two inactive fragments of Cre are reconstituted to form a functionally active enzyme in the presence of rapamycin. Different loci have been targeted to generate parasite lines that express the DiCre enzyme. Here, we have used marker-free CRISPR-Cas9 gene editing to integrate the DiCre cassette in a redundant cg6 locus. We have shown the utility of the newly generated ∆cg6DC4 parasites in mediating robust, rapid, and highly specific excision of exogenously encoded gfp sequence. The ∆cg6DC4 parasites are also capable of conditional e","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Oct","modification":"2025-04-04T02:19:56.149Z","creation":"2025-04-04T02:19:56.149Z"},"accession":"S-EPMC11473785","cross_references":{"pubmed":["39402380"],"doi":["10.1038/s41598-024-75657-x"]}}