biostudies-literatureUnknownXu JNatural Science Foundation of Hunan ProvinceNational Natural Science Foundation of ChinaNanhu Scholars Program for Young Scholars of Xinyang Normal UniversityXinyang Normal UniversityA bidirectional self-powered biosensor is constructed for the quasi-simultaneous detection of Pb²⁺ and Hg²⁺ based on MoS₂@CuS heterostructures as an accelerator and hybridization chain reaction (HCR) as a signal amplification strategy. MoS₂@CuS heterostructures significantly facilitate electron transfer between glucose and bioelectrodes, thereby greatly improving the detection signal of self-powered biosensors. This novel biosensor employs the unique sequences of DNAzymes to isolate Pb²⁺ and Hg²⁺ by the cleavage effect and thymine (T)-Hg²⁺-thymine (T) structures, respectively. In the process, Pb²⁺ cuts the sequence of DNAzyme at the bioanode to trigger glucose oxidation to monitor Pb²⁺. The aChemical sciencehttps://www.ebi.ac.uk/biostudies/studies/S-EPMC11474792biostudies-literatureOn-demand controlled bidirectional DNAzyme path for ultra-sensitive heavy metal ion detection.PMC114747922021JJ20020220040322024JJ5348Zhang WYang HCai RLi YTan WLyu YWang FLuo XXu JfalseOn-demand controlled bidirectional DNAzyme path for ultra-sensitive heavy metal ion detection.A bidirectional self-powered biosensor is constructed for the quasi-simultaneous detection of Pb²⁺ and Hg²⁺ based on MoS₂@CuS heterostructures as an accelerator and hybridization chain reaction (HCR) as a signal amplification strategy. MoS₂@CuS heterostructures significantly facilitate electron transfer between glucose and bioelectrodes, thereby greatly improving the detection signal of self-powered biosensors. This novel biosensor employs the unique sequences of DNAzymes to isolate Pb²⁺ and Hg²⁺ by the cleavage effect and thymine (T)-Hg²⁺-thymine (T) structures, respectively. In the process, Pb²⁺ cuts the sequence of DNAzyme at the bioanode to trigger glucose oxidation to monitor Pb²⁺. The a2024-01-01T00:00:00Z2024 Oct2025-04-04T00:08:31.492Z2025-04-04T00:08:31.492ZS-EPMC114747923942120610.1039/d4sc04404e