{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Sui S"],"funding":["Fundamental Research Funds for the Central Universities","Natural Science Foundation of China","Pearl River Talents Scheme of Guangdong Province"],"pagination":["124"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11476310"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(1)"],"pubmed_abstract":["Background
Exhaustion is a key factor that influences the efficacy of chimeric antigen receptor T (CAR-T) cells. Our previous study demonstrated that a bromodomain protein 4 (BRD4) inhibitor can revise the phenotype and function of exhausted T cells from leukemia patients. This study aims to elucidate the mechanism by which a BRD4 inhibitor reduces CAR-T cell exhaustion using single-cell RNA sequencing (scRNA-Seq).Methods
Exhausted CD123-specific CAR-T cells were prepared by co-culture with CD123 antigen-positive MV411 cells. After elimination of MV411 cells and upregulation of inhibitory receptors on the surface, exhausted CAR-T cells were treated with a BRD4 inhibitor (JQ1) for 72 h. The CAR-T cells were subsequently isolated, and scRNA-Seq was conducted to characterize p"],"journal":["Biomarker research"],"pubmed_title":["BRD4 inhibitor reduces exhaustion and blocks terminal differentiation in CAR-T cells by modulating BATF and EGR1."],"pmcid":["PMC11476310"],"funding_grant_id":["21623121","2019QN01Y990","82293632","81971301"],"pubmed_authors":["Peng X","Zeng C","Luo OJ","Sui S","Li Y","Zhong S","Chen C","Mao L","Zhong M"],"additional_accession":[]},"is_claimable":false,"name":"BRD4 inhibitor reduces exhaustion and blocks terminal differentiation in CAR-T cells by modulating BATF and EGR1.","description":"Background
Exhaustion is a key factor that influences the efficacy of chimeric antigen receptor T (CAR-T) cells. Our previous study demonstrated that a bromodomain protein 4 (BRD4) inhibitor can revise the phenotype and function of exhausted T cells from leukemia patients. This study aims to elucidate the mechanism by which a BRD4 inhibitor reduces CAR-T cell exhaustion using single-cell RNA sequencing (scRNA-Seq).Methods
Exhausted CD123-specific CAR-T cells were prepared by co-culture with CD123 antigen-positive MV411 cells. After elimination of MV411 cells and upregulation of inhibitory receptors on the surface, exhausted CAR-T cells were treated with a BRD4 inhibitor (JQ1) for 72 h. The CAR-T cells were subsequently isolated, and scRNA-Seq was conducted to characterize p","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Oct","modification":"2026-07-15T13:07:22.982Z","creation":"2025-04-04T07:41:13.266Z"},"accession":"S-EPMC11476310","cross_references":{"pubmed":["39407311"],"doi":["10.1186/s40364-024-00667-w"]}}