biostudies-literatureXie XNational Agriculture Science and Technology Major ProgramNational Key Research and Development Program of Chinathe National Natural Science Foundation of China964https://www.ebi.ac.uk/biostudies/studies/S-EPMC11476473biostudies-literatureUnknown24(1)The southern root-knot nematode, Meloidogyne incognita, is a highly serious plant parasitic nematode species that causes significant economic losses in various crops, including cucumber (Cucumis sativus L.). Currently, there are no commercial cultivars available with resistance to M. incognita in cucumber. However, the African horned melon (Cucumis metuliferus Naud.), a semi-wild relative of cucumber, has shown high resistance to M. incognita. In this study, we constructed an ultrahigh-density genetic linkage bin-map using low-coverage sequences from an F₂ population generated through the cross between C. metuliferus inbred lines CM3 and CM27. Finally, we identified a QTL (quantitative trait locus, QTL3.1) with a LOD (logarithm of the odds) score of 3.84, explaining 8.4% of the BMC plant biologyGenetic dissection of Meloidogyne incognita resistance genes based on VIGS functional analysis in Cucumis metuliferus.PMC114764732023YFD1400400NK202209043237250831571996Visser RGFLi YXie XLu JZheng SLing JYang QBai YZhao JMao ZXie BfalseGenetic dissection of Meloidogyne incognita resistance genes based on VIGS functional analysis in Cucumis metuliferus.The southern root-knot nematode, Meloidogyne incognita, is a highly serious plant parasitic nematode species that causes significant economic losses in various crops, including cucumber (Cucumis sativus L.). Currently, there are no commercial cultivars available with resistance to M. incognita in cucumber. However, the African horned melon (Cucumis metuliferus Naud.), a semi-wild relative of cucumber, has shown high resistance to M. incognita. In this study, we constructed an ultrahigh-density genetic linkage bin-map using low-coverage sequences from an F₂ population generated through the cross between C. metuliferus inbred lines CM3 and CM27. Finally, we identified a QTL (quantitative trait locus, QTL3.1) with a LOD (logarithm of the odds) score of 3.84, explaining 8.4% of the 2024-01-01T00:00:00Z2024 Oct2025-04-04T00:35:49.721Z2025-04-04T00:35:49.721ZS-EPMC114764733940244610.1186/s12870-024-05681-6