biostudies-literatureThuy PXMinistry of Science and ICTNational Research Foundation of Korea (NRF)Basic Research (BR) through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICTBasic Research (BR) Laboratory Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT10369https://www.ebi.ac.uk/biostudies/studies/S-EPMC11477320biostudies-literatureUnknown25(19)Primary cilia (PC) are microtubule-based organelles that function as cellular antennae to sense and transduce extracellular signals. Nephronophthisis 3 (NPHP3) is localized in the inversin compartment of PC. Mutations in NPHP3 are associated with renal-hepatic-pancreatic dysplasia. In this study, we investigated whether vinblastine (VBL), a microtubule destabilizer, induces anticancer drug resistance through NPHP3-associated PC formation in HeLa human cervical cancer cells. A considerable increase in PC frequency was observed in HeLa cells under serum-deprived (SD) conditions, which led to the inhibition of VBL-induced cell death. VBL-resistant cells were established by repetitive treatments with VBL and showed an increase in PC frequency. NPHP3 expression was also increased by VBL treatmeInternational journal of molecular sciencesVinblastine Resistance Is Associated with Nephronophthisis 3-Mediated Primary Cilia via Intraflagellar Transport Protein 88 and Apoptosis-Antagonizing Transcription Factor.PMC114773202021R1A4A5033289RS-2023-00244570Jang TKThuy PXMoon EYfalseVinblastine Resistance Is Associated with Nephronophthisis 3-Mediated Primary Cilia via Intraflagellar Transport Protein 88 and Apoptosis-Antagonizing Transcription Factor.Primary cilia (PC) are microtubule-based organelles that function as cellular antennae to sense and transduce extracellular signals. Nephronophthisis 3 (NPHP3) is localized in the inversin compartment of PC. Mutations in NPHP3 are associated with renal-hepatic-pancreatic dysplasia. In this study, we investigated whether vinblastine (VBL), a microtubule destabilizer, induces anticancer drug resistance through NPHP3-associated PC formation in HeLa human cervical cancer cells. A considerable increase in PC frequency was observed in HeLa cells under serum-deprived (SD) conditions, which led to the inhibition of VBL-induced cell death. VBL-resistant cells were established by repetitive treatments with VBL and showed an increase in PC frequency. NPHP3 expression was also increased by VBL treatme2024-01-01T00:00:00Z2024 Sep2026-07-02T03:15:28.037Z2025-04-04T00:35:02.144ZS-EPMC114773203940870110.3390/ijms251910369