{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Vorvi S"],"funding":["Horizon 2020-EU"],"pagination":["4736"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11477641"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["29(19)"],"pubmed_abstract":["The enhanced and direct immobilization of the enzyme horseradish peroxidase on poly(methyl methacrylate) (PMMA) microchannel surfaces to create a miniaturized enzymatic reactor for the biocatalytic oxidation of phenols is demonstrated. Enzyme immobilization occurs by physical adsorption after oxygen plasma treatment, which micro-nanotextures the PMMA surfaces. A five-fold enhancement in immobilized enzyme activity was observed, attributed to the increased surface area and, therefore, to a higher quantity of immobilized enzymes compared to an untreated PMMA surface. The enzymatic reaction yield reached 75% using a flow rate of 2.0 μL/min for the reaction mixture. Additionally, the developed microreactor was reused more than 16 times without affecting the enzymatic conversion yield. These re"],"journal":["Molecules (Basel, Switzerland)"],"pubmed_title":["Enhanced Immobilization of Enzymes on Plasma Micro-Nanotextured Surfaces and Microfluidics: Application to HRP."],"pmcid":["PMC11477641"],"funding_grant_id":["68768"],"pubmed_authors":["Vorvi S","Tsougeni K","Gogolides E","Kakabakos S","Petrou P","Tserepi A"],"additional_accession":[]},"is_claimable":false,"name":"Enhanced Immobilization of Enzymes on Plasma Micro-Nanotextured Surfaces and Microfluidics: Application to HRP.","description":"The enhanced and direct immobilization of the enzyme horseradish peroxidase on poly(methyl methacrylate) (PMMA) microchannel surfaces to create a miniaturized enzymatic reactor for the biocatalytic oxidation of phenols is demonstrated. Enzyme immobilization occurs by physical adsorption after oxygen plasma treatment, which micro-nanotextures the PMMA surfaces. A five-fold enhancement in immobilized enzyme activity was observed, attributed to the increased surface area and, therefore, to a higher quantity of immobilized enzymes compared to an untreated PMMA surface. The enzymatic reaction yield reached 75% using a flow rate of 2.0 μL/min for the reaction mixture. Additionally, the developed microreactor was reused more than 16 times without affecting the enzymatic conversion yield. These re","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Oct","modification":"2025-04-22T10:51:16.234Z","creation":"2025-04-05T23:46:03.126Z"},"accession":"S-EPMC11477641","cross_references":{"pubmed":["39407664"],"doi":["10.3390/molecules29194736"]}}