{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Sendinc E"],"funding":["NHGRI NIH HHS","NCI NIH HHS"],"pubmed_abstract":["RNA is subject to a multitude of different chemical modifications that collectively represent the epitranscriptome. Individual RNA modifications including N6-methyladenosine (m<sup>6</sup>A) on mRNA play essential roles in the posttranscriptional control of gene expression. Recent technological advances have enabled the transcriptome-wide mapping of certain RNA modifications, to reveal their broad relevance and characteristic distribution patterns. However, convenient methods that enable the simultaneous mapping of multiple different RNA marks within the same sample are generally lacking. Here we present EpiPlex RNA modification profiling, a bead-based proximity barcoding assay with sequencing readout that expands the scope of molecular recognition-based RNA modification detection to multi"],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2024.10.09.617509"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11482933"],"repository":["biostudies-literature"],"pubmed_title":["Mapping multiple RNA modifications simultaneously by proximity barcode sequencing."],"pmcid":["PMC11482933"],"funding_grant_id":["R44 HG012170","R35 CA232115","R43 HG012170"],"pubmed_authors":["Anmangandla A","Davis EM","Cantu VA","Chabot MB","Sendinc E","Price AD","Burcham TS","Santos J","Yu H","Johnson Z","Miles ZD","Purse BW","Kirstein JR","Lam Q","Gregory RI","Stengel G","Dzakula Z","Niu J","Hwang Fu YH"],"additional_accession":[]},"is_claimable":false,"name":"Mapping multiple RNA modifications simultaneously by proximity barcode sequencing.","description":"RNA is subject to a multitude of different chemical modifications that collectively represent the epitranscriptome. Individual RNA modifications including N6-methyladenosine (m<sup>6</sup>A) on mRNA play essential roles in the posttranscriptional control of gene expression. Recent technological advances have enabled the transcriptome-wide mapping of certain RNA modifications, to reveal their broad relevance and characteristic distribution patterns. However, convenient methods that enable the simultaneous mapping of multiple different RNA marks within the same sample are generally lacking. Here we present EpiPlex RNA modification profiling, a bead-based proximity barcoding assay with sequencing readout that expands the scope of molecular recognition-based RNA modification detection to multi","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Oct","modification":"2026-07-09T10:26:30.659Z","creation":"2025-04-06T22:45:09.202Z"},"accession":"S-EPMC11482933","cross_references":{"pubmed":["39416026"],"doi":["10.1101/2024.10.09.617509"]}}