{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["16(1)"],"submitter":["Fargeas M"],"funding":["Agence Nationale de la Recherche"],"pubmed_abstract":["We investigated the role of ChiA and its associated polymorphisms in the interaction between Crohn's disease (CD)-associated adherent-invasive <i>Escherichia coli</i> (AIEC) and intestinal mucosa. We observed a higher abundance of <i>chiA</i> among the metagenome of CD patients compared to healthy subjects. In dextran sulfate sodium-induced colitis mice model, AIEC-LF82∆<i>chiA</i> colonization was reduced in ileal, colonic and fecal samples compared to wild-type LF82. The binding of ChiA to recombinant human CHI3L1 or mucus was higher with the pathogenic polymorphism. The strength of ChiA-mucin interaction was 300-fold stronger than ChiA-rhCHI3L1. ChiA was able to degrade mucin to promote its growth and enabled LF82 to get closer to epithelial cells. The pathogenic polymorphism of ChiA ha"],"journal":["Gut microbes"],"pagination":["2412667"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11486038"],"repository":["biostudies-literature"],"pubmed_title":["ChiA: a major player in the virulence of Crohn's disease-associated adherent and invasive <i>Escherichia coli</i> (AIEC)."],"pmcid":["PMC11486038"],"pubmed_authors":["Faure F","Denizot J","Buisson A","Chevarin C","Birer A","Billard E","Barnich N","Fargeas M","Rodrigues M","Douadi C","Sivignon A"],"additional_accession":[]},"is_claimable":false,"name":"ChiA: a major player in the virulence of Crohn's disease-associated adherent and invasive <i>Escherichia coli</i> (AIEC).","description":"We investigated the role of ChiA and its associated polymorphisms in the interaction between Crohn's disease (CD)-associated adherent-invasive <i>Escherichia coli</i> (AIEC) and intestinal mucosa. We observed a higher abundance of <i>chiA</i> among the metagenome of CD patients compared to healthy subjects. In dextran sulfate sodium-induced colitis mice model, AIEC-LF82∆<i>chiA</i> colonization was reduced in ileal, colonic and fecal samples compared to wild-type LF82. The binding of ChiA to recombinant human CHI3L1 or mucus was higher with the pathogenic polymorphism. The strength of ChiA-mucin interaction was 300-fold stronger than ChiA-rhCHI3L1. ChiA was able to degrade mucin to promote its growth and enabled LF82 to get closer to epithelial cells. The pathogenic polymorphism of ChiA ha","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Jan-Dec","modification":"2026-07-01T03:10:19.683Z","creation":"2025-04-04T01:54:06.726Z"},"accession":"S-EPMC11486038","cross_references":{"pubmed":["39397494"],"doi":["10.1080/19490976.2024.2412667"]}}