biostudies-literatureUnknown16(1)Fargeas MAgence Nationale de la RechercheWe investigated the role of ChiA and its associated polymorphisms in the interaction between Crohn's disease (CD)-associated adherent-invasive <i>Escherichia coli</i> (AIEC) and intestinal mucosa. We observed a higher abundance of <i>chiA</i> among the metagenome of CD patients compared to healthy subjects. In dextran sulfate sodium-induced colitis mice model, AIEC-LF82∆<i>chiA</i> colonization was reduced in ileal, colonic and fecal samples compared to wild-type LF82. The binding of ChiA to recombinant human CHI3L1 or mucus was higher with the pathogenic polymorphism. The strength of ChiA-mucin interaction was 300-fold stronger than ChiA-rhCHI3L1. ChiA was able to degrade mucin to promote its growth and enabled LF82 to get closer to epithelial cells. The pathogenic polymorphism of ChiA haGut microbes2412667https://www.ebi.ac.uk/biostudies/studies/S-EPMC11486038biostudies-literatureChiA: a major player in the virulence of Crohn's disease-associated adherent and invasive <i>Escherichia coli</i> (AIEC).PMC11486038Faure FDenizot JBuisson AChevarin CBirer ABillard EBarnich NFargeas MRodrigues MDouadi CSivignon AfalseChiA: a major player in the virulence of Crohn's disease-associated adherent and invasive <i>Escherichia coli</i> (AIEC).We investigated the role of ChiA and its associated polymorphisms in the interaction between Crohn's disease (CD)-associated adherent-invasive <i>Escherichia coli</i> (AIEC) and intestinal mucosa. We observed a higher abundance of <i>chiA</i> among the metagenome of CD patients compared to healthy subjects. In dextran sulfate sodium-induced colitis mice model, AIEC-LF82∆<i>chiA</i> colonization was reduced in ileal, colonic and fecal samples compared to wild-type LF82. The binding of ChiA to recombinant human CHI3L1 or mucus was higher with the pathogenic polymorphism. The strength of ChiA-mucin interaction was 300-fold stronger than ChiA-rhCHI3L1. ChiA was able to degrade mucin to promote its growth and enabled LF82 to get closer to epithelial cells. The pathogenic polymorphism of ChiA ha2024-01-01T00:00:00Z2024 Jan-Dec2026-07-01T03:10:19.683Z2025-04-04T01:54:06.726ZS-EPMC114860383939749410.1080/19490976.2024.2412667