biostudies-literatureUnknown35(3)Li PMicroRNAs (miRNAs) are present in serum and have the potential to serve as disease biomarkers. As such, it is important to explore the clinical value of miRNAs in serum as biomarkers for ischemic stroke (IS) and cast light on the pathogenesis of IS. In this study, we screened differentially expressed serum miRNAs from IS and normal people by miRNA microarray analysis, and validated the expression of candidate miRNAs using quantitative reverse-transcriptase polymerase chain reaction assays. Furthermore, we performed gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) pathway analyses to disclose functional enrichment of genes predicted to be regulated by the differentially expressed miRNAs. Notably, our results revealed that 115 miRNAs were differentially expressed in IS, Cellular and molecular neurobiology433-447https://www.ebi.ac.uk/biostudies/studies/S-EPMC11486203biostudies-literatureIdentification of circulating microRNAs as potential biomarkers for detecting acute ischemic stroke.PMC11486203Wu JLi PTeng FZhang MZhang CGao FfalseIdentification of circulating microRNAs as potential biomarkers for detecting acute ischemic stroke.MicroRNAs (miRNAs) are present in serum and have the potential to serve as disease biomarkers. As such, it is important to explore the clinical value of miRNAs in serum as biomarkers for ischemic stroke (IS) and cast light on the pathogenesis of IS. In this study, we screened differentially expressed serum miRNAs from IS and normal people by miRNA microarray analysis, and validated the expression of candidate miRNAs using quantitative reverse-transcriptase polymerase chain reaction assays. Furthermore, we performed gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) pathway analyses to disclose functional enrichment of genes predicted to be regulated by the differentially expressed miRNAs. Notably, our results revealed that 115 miRNAs were differentially expressed in IS, 2015-01-01T00:00:00Z2015 Apr2026-06-02T00:00:33.116Z2025-04-04T20:24:58.88ZS-EPMC114862032541030410.1007/s10571-014-0139-5