{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Le Pen J"],"funding":["Swiss National Science Foundation","NCATS NIH HHS","NIAID NIH HHS"],"pagination":["e3002767"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11486371"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["22(9)"],"pubmed_abstract":["Interferons (IFNs) play a crucial role in the regulation and evolution of host-virus interactions. Here, we conducted a genome-wide arrayed CRISPR knockout screen in the presence and absence of IFN to identify human genes that influence Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection. We then performed an integrated analysis of genes interacting with SARS-CoV-2, drawing from a selection of 67 large-scale studies, including our own. We identified 28 genes of high relevance in both human genetic studies of Coronavirus Disease 2019 (COVID-19) patients and functional genetic screens in cell culture, with many related to the IFN pathway. Among these was the IFN-stimulated gene PLSCR1. PLSCR1 did not require IFN induction to restrict SARS-CoV-2 and did not contribute to IF"],"journal":["PLoS biology"],"pubmed_title":["A genome-wide arrayed CRISPR screen identifies PLSCR1 as an intrinsic barrier to SARS-CoV-2 entry that recent virus variants have evolved to resist."],"pmcid":["PMC11486371"],"funding_grant_id":["R01 AI091707","U19 AI111825","R01 AI163029","R01 AI161444","UL1 TR001866","203007"],"pubmed_authors":["MacDonald MR","Dinnon KH","Ricardo-Lax I","Schmidt C","Pinharanda A","McGinn T","Paniccia G","Lee D","Zhang Q","Carroll TS","Moncada-Velez M","Yip EZ","Bauer M","Freije CA","Sanchez-Rivera FJ","Cobat A","Razooky B","Lewy T","Doyle WC","Ashbrook AW","Banerjee P","Kinast V","Stenzel AF","Rosado-Olivieri EA","Schneider WM","Ramos-Espiritu L","Casanova JL","Menezes MR","Poirier JT","Hoffmann HH","Michailidis E","Le Pen J","Luna JM","Rice CM","Hong SH","Glickman JF","Peace A","Winkler R","Larson C"],"additional_accession":[]},"is_claimable":false,"name":"A genome-wide arrayed CRISPR screen identifies PLSCR1 as an intrinsic barrier to SARS-CoV-2 entry that recent virus variants have evolved to resist.","description":"Interferons (IFNs) play a crucial role in the regulation and evolution of host-virus interactions. Here, we conducted a genome-wide arrayed CRISPR knockout screen in the presence and absence of IFN to identify human genes that influence Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection. We then performed an integrated analysis of genes interacting with SARS-CoV-2, drawing from a selection of 67 large-scale studies, including our own. We identified 28 genes of high relevance in both human genetic studies of Coronavirus Disease 2019 (COVID-19) patients and functional genetic screens in cell culture, with many related to the IFN pathway. Among these was the IFN-stimulated gene PLSCR1. PLSCR1 did not require IFN induction to restrict SARS-CoV-2 and did not contribute to IF","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Sep","modification":"2026-07-15T09:36:31.074Z","creation":"2025-04-06T09:33:54.991Z"},"accession":"S-EPMC11486371","cross_references":{"pubmed":["39316623"],"doi":["10.1371/journal.pbio.3002767"]}}