biostudies-literatureUnknown15Fan Z<h4>Introduction</h4>Corticotomy-assisted tooth movement is commonly performed in clinics, however, its time-limited efficacy and the fear of surgery among patients significantly limit its clinical application. Hence, researchers have investigated non-invasive methods to accelerate tooth movement. However, the molecular mechanisms underlying corticotomy-assisted tooth movement are not fully understood.<h4>Methods</h4>Micro-CT and TRAP stain were used to tooth movement and bone resorption. Single-cell RNA sequencing was used to study the transcriptome heterogeneity of macrophages after corticotomy. Transmission electron microscopy and iron ion detection was used to evaluate ferroptosis and iron metabolism. In addition, we carried out immunohistochemistry, quantitative real-time and flow cytFrontiers in immunology1397727https://www.ebi.ac.uk/biostudies/studies/S-EPMC11487196biostudies-literatureSingle-cell sequencing decodes the secrets of the RAP phenomenon of corticotomy.PMC11487196You LLi SFan ZMa YZhong YXu XLan YXu JfalseSingle-cell sequencing decodes the secrets of the RAP phenomenon of corticotomy.<h4>Introduction</h4>Corticotomy-assisted tooth movement is commonly performed in clinics, however, its time-limited efficacy and the fear of surgery among patients significantly limit its clinical application. Hence, researchers have investigated non-invasive methods to accelerate tooth movement. However, the molecular mechanisms underlying corticotomy-assisted tooth movement are not fully understood.<h4>Methods</h4>Micro-CT and TRAP stain were used to tooth movement and bone resorption. Single-cell RNA sequencing was used to study the transcriptome heterogeneity of macrophages after corticotomy. Transmission electron microscopy and iron ion detection was used to evaluate ferroptosis and iron metabolism. In addition, we carried out immunohistochemistry, quantitative real-time and flow cyt2024-01-01T00:00:00Z20242025-04-04T01:10:45.885Z2025-04-04T01:10:45.885ZS-EPMC114871963943074710.3389/fimmu.2024.1397727