{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["4(5)"],"submitter":["Al-Hawat ML"],"pubmed_abstract":["Ammonia is a key biomarker in inborn and acquired liver disease. As clinical point-of-care blood ammonia assays are lacking, we developed a polymersome formulation for point-of-care blood ammonia sensing combined with a portable fluorometer. A pH-sensitive near-infrared (NIR) fluorescent dye was identified, which showed a strong fluorescence increase at acidic pH values. Building on reports on ammonia-selective PS-<i>b</i>-PEG polymersomes, these polymersomes were loaded with the NIR dye. These NIR fluorescent polymersomes sensed ammonia in a clinically relevant range in ammonia-spiked fresh whole blood with high linearity (<i>R</i> <sup>2</sup> = 0.9948) after 5 min using a conventional tabletop plate reader. Subsequently, the assay was tested with a portable fluorometer. An ammonia-dependent fluorescence increase was detected in ammonia-spiked fresh mouse blood after 5 min using the portable fluorometer. The NIR dye-loaded PS-<i>b</i>-PEG polymersomes rapidly sensed ammonia with high linearity in whole blood. This assay was successfully combined with a portable fluorometer and only required 3 μL of blood. These findings motivate a further development and clinical translation of this point-of-care blood ammonia assay."],"journal":["ACS bio & med chem Au"],"pagination":["226-232"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11487535"],"repository":["biostudies-literature"],"pubmed_title":["Development of a Polymersome Blood Ammonia Assay Coupled with a Portable Near-Infrared Fluorometer."],"pmcid":["PMC11487535"],"pubmed_authors":["Al-Hawat ML","Djebbar S","Caron J","Matoori S"],"additional_accession":[]},"is_claimable":false,"name":"Development of a Polymersome Blood Ammonia Assay Coupled with a Portable Near-Infrared Fluorometer.","description":"Ammonia is a key biomarker in inborn and acquired liver disease. As clinical point-of-care blood ammonia assays are lacking, we developed a polymersome formulation for point-of-care blood ammonia sensing combined with a portable fluorometer. A pH-sensitive near-infrared (NIR) fluorescent dye was identified, which showed a strong fluorescence increase at acidic pH values. Building on reports on ammonia-selective PS-<i>b</i>-PEG polymersomes, these polymersomes were loaded with the NIR dye. These NIR fluorescent polymersomes sensed ammonia in a clinically relevant range in ammonia-spiked fresh whole blood with high linearity (<i>R</i> <sup>2</sup> = 0.9948) after 5 min using a conventional tabletop plate reader. Subsequently, the assay was tested with a portable fluorometer. An ammonia-dependent fluorescence increase was detected in ammonia-spiked fresh mouse blood after 5 min using the portable fluorometer. The NIR dye-loaded PS-<i>b</i>-PEG polymersomes rapidly sensed ammonia with high linearity in whole blood. This assay was successfully combined with a portable fluorometer and only required 3 μL of blood. These findings motivate a further development and clinical translation of this point-of-care blood ammonia assay.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Oct","modification":"2025-04-04T20:55:56.845Z","creation":"2025-04-04T20:55:56.845Z"},"accession":"S-EPMC11487535","cross_references":{"pubmed":["39431266"],"doi":["10.1021/acsbiomedchemau.4c00013"]}}