biostudies-literatureUnknown3(5)Lu YXHi-C can obtain three-dimensional chromatin structure information and is widely used for genome assembly. We constructed the GutHi-C technology. As shown in the graphical abstract, it is a highly efficient and quick-to-operate method and can be widely used for human, livestock, and poultry gut microorganisms. It provides a reference for the Hi-C methodology of the microbial metagenome. DPBS, Dulbecco's phosphate-buffered saline; Hi-C, high-through chromatin conformation capture; LB, Luria-Bertani; NGS, next-generation sequencing; PCR, polymerase chain reaction; QC, quality control.iMetae227https://www.ebi.ac.uk/biostudies/studies/S-EPMC11487548biostudies-literatureEfficient and easy-to-use capturing three-dimensional metagenome interactions with GutHi-C.PMC11487548Chang RRLu YXKong SYLi CYYang SLZhu XSKong DSZhang YBYang JBPan WHWang YFTian YHfalseEfficient and easy-to-use capturing three-dimensional metagenome interactions with GutHi-C.Hi-C can obtain three-dimensional chromatin structure information and is widely used for genome assembly. We constructed the GutHi-C technology. As shown in the graphical abstract, it is a highly efficient and quick-to-operate method and can be widely used for human, livestock, and poultry gut microorganisms. It provides a reference for the Hi-C methodology of the microbial metagenome. DPBS, Dulbecco's phosphate-buffered saline; Hi-C, high-through chromatin conformation capture; LB, Luria-Bertani; NGS, next-generation sequencing; PCR, polymerase chain reaction; QC, quality control.2024-01-01T00:00:00Z2024 Oct2025-04-04T01:12:02.027Z2025-04-04T01:12:02.027ZS-EPMC114875483942987910.1002/imt2.227