biostudies-literatureShi Zthe Xinjiang Tianshan Talent Training Program Grantthe National Natural Science Foundation of China regional projectthe Xinjiang Natural Science Foundation for Distinguished Young Scholars179https://www.ebi.ac.uk/biostudies/studies/S-EPMC11488200biostudies-literatureUnknown30(1)<h4>Background</h4>Docetaxel (DTX) resistance attenuates anti-tumor effects of DTX on prostate cancer (mCRPC) and drug resistance was related to Treg expansion in tumors. ZNF667-AS1 played a suppressing role in various tumors and tumor-derived exosomes carry lncRNAs to participate in tumor progression. Here, the effects of ZNF667-AS1 on malignant characteristics and DTX resistance in PC and the effect and its underlying molecular mechanism of tumor-derived exosomes carrying ZNF667-AS1 on Treg expansion were investigated.<h4>Methods</h4>The identification of exosomes were determined using TEM, NTA and western blot. The abundance of genes and proteins were evaluated using IHC, RT-qPCR, western blot and FISH. Malignant phenotypes of PC cells were evaluated by means of Edu, scratch test, transMolecular medicine (Cambridge, Mass.)Prostate cancer cell-derived exosomes ZNF667-AS1 reduces TGFBR1 mRNA stability to inhibit Treg expansion and DTX resistance by binding to U2AF1.PMC11488200823605202023TSYCCX00612022D01E84Sun MLi JLi ZAihemaitijiang MLi SZhang XPu WLiu BShi ZLi MfalseProstate cancer cell-derived exosomes ZNF667-AS1 reduces TGFBR1 mRNA stability to inhibit Treg expansion and DTX resistance by binding to U2AF1.<h4>Background</h4>Docetaxel (DTX) resistance attenuates anti-tumor effects of DTX on prostate cancer (mCRPC) and drug resistance was related to Treg expansion in tumors. ZNF667-AS1 played a suppressing role in various tumors and tumor-derived exosomes carry lncRNAs to participate in tumor progression. Here, the effects of ZNF667-AS1 on malignant characteristics and DTX resistance in PC and the effect and its underlying molecular mechanism of tumor-derived exosomes carrying ZNF667-AS1 on Treg expansion were investigated.<h4>Methods</h4>The identification of exosomes were determined using TEM, NTA and western blot. The abundance of genes and proteins were evaluated using IHC, RT-qPCR, western blot and FISH. Malignant phenotypes of PC cells were evaluated by means of Edu, scratch test, trans2024-01-01T00:00:00Z2024 Oct2025-04-04T02:21:16.075Z2025-04-04T02:21:16.075ZS-EPMC114882003942500910.1186/s10020-024-00947-z