biostudies-literatureLu L“HuiLi Funded” Research ProjectNatural Science Foundation of NingboThe Medical and Health Plan of ZhejiangMedical Science and Technology Project of Ningbo"HuiLi Funded" Research Project180https://www.ebi.ac.uk/biostudies/studies/S-EPMC11488288biostudies-literatureUnknown30(1)This study investigates the potential molecular mechanisms by which O-GlcNAc modification of YTHDF2 regulates the cell cycle and participates in intervertebral disc degeneration (IDD). We employed transcriptome sequencing to identify genes involved in IDD and utilized bioinformatics analysis to predict key disease-related genes. In vitro mechanistic validation was performed using mouse nucleus pulposus (NP) cells. Changes in reactive oxygen species (ROS) and cell cycle were assessed through flow cytometry and CCK-8 assays. An IDD mouse model was also established for in vivo mechanistic validation, with changes in IDD severity measured using X-rays and immunohistochemical staining. Bioinformatics analysis revealed differential expression of YTHDF2 in NP cells of normal and IDD mice, suggestMolecular medicine (Cambridge, Mass.)New perspectives on YTHDF2 O-GlcNAc modification in the pathogenesis of intervertebral disc degeneration.PMC11488288No.2023KY1025Grant No.2022Y022022YB009No.2023J032Lu LYang MWang HWang LfalseNew perspectives on YTHDF2 O-GlcNAc modification in the pathogenesis of intervertebral disc degeneration.This study investigates the potential molecular mechanisms by which O-GlcNAc modification of YTHDF2 regulates the cell cycle and participates in intervertebral disc degeneration (IDD). We employed transcriptome sequencing to identify genes involved in IDD and utilized bioinformatics analysis to predict key disease-related genes. In vitro mechanistic validation was performed using mouse nucleus pulposus (NP) cells. Changes in reactive oxygen species (ROS) and cell cycle were assessed through flow cytometry and CCK-8 assays. An IDD mouse model was also established for in vivo mechanistic validation, with changes in IDD severity measured using X-rays and immunohistochemical staining. Bioinformatics analysis revealed differential expression of YTHDF2 in NP cells of normal and IDD mice, suggest2024-01-01T00:00:00Z2024 Oct2026-07-15T05:38:09.674Z2025-04-04T01:50:57.178ZS-EPMC114882883942501310.1186/s10020-024-00876-x