biostudies-literatureWu SHMinistry of Science and Technology, TaiwanTaipei Veterans General HospitalMinistry of Health and WelfareTaoyuan General Hospital837-849https://www.ebi.ac.uk/biostudies/studies/S-EPMC11488484biostudies-literatureUnknown26We compared infant bone marrow-derived mesenchymal stem cells (infant BMSCs) with umbilical cord-derived mesenchymal stem cells (UCSCs) by assessing multilineage differentiation. Proliferation was gauged through changes in cell numbers and doubling time. Senescence-related genes (<i>p16</i>, <i>p21</i>, and <i>p53</i>), senescence-associated β-galactosidase (SA-β-gal), and γH2AX immunofluorescence determined senescence presence. Superoxide dismutases (SODs) and genes related to various differentiations were analyzed using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Differentiation was confirmed through histochemical, immunohistochemical, and immunofluorescence staining. Infant BMSCs surpassed UCSCs in proliferation. Infant BMSCs exhibited lower senescence-relateRegenerative therapyComparison of infant bone marrow- and umbilical cord-derived mesenchymal stem cells in multilineage differentiation.PMC11488484V111C-231111-2314-B-075-057-MY3PTH111063Liao YTWang JPHsueh KKChou PHWu SHYu JHWen MHfalseComparison of infant bone marrow- and umbilical cord-derived mesenchymal stem cells in multilineage differentiation.We compared infant bone marrow-derived mesenchymal stem cells (infant BMSCs) with umbilical cord-derived mesenchymal stem cells (UCSCs) by assessing multilineage differentiation. Proliferation was gauged through changes in cell numbers and doubling time. Senescence-related genes (<i>p16</i>, <i>p21</i>, and <i>p53</i>), senescence-associated β-galactosidase (SA-β-gal), and γH2AX immunofluorescence determined senescence presence. Superoxide dismutases (SODs) and genes related to various differentiations were analyzed using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Differentiation was confirmed through histochemical, immunohistochemical, and immunofluorescence staining. Infant BMSCs surpassed UCSCs in proliferation. Infant BMSCs exhibited lower senescence-relate2024-01-01T00:00:00Z2024 Jun2026-06-24T03:10:09.749Z2025-04-04T19:46:02.936ZS-EPMC114884843943058010.1016/j.reth.2024.09.011