{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Mangilet AF"],"funding":["Deutsche Forschungsgemeinschaft","Deutsche Forschungsgemeinschaft (German Research Foundation)"],"pagination":["1514-1531"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11489095"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["10(10)"],"pubmed_abstract":["The removal of introns by the spliceosome is a key gene regulatory mechanism in eukaryotes, with the U1 snRNP subunit playing a crucial role in the early stages of splicing. Studies in metazoans show that the U1 snRNP also conducts splicing-independent functions, but the lack of genetic tools and knowledge about U1 snRNP-associated proteins have limited the study of such splicing-independent functions in plants. Here we describe an RNA-centric approach that identified more than 200 proteins associated with the Arabidopsis U1 snRNP and revealed a tight link to mRNA cleavage and polyadenylation factors. Interestingly, we found that the U1 snRNP protects mRNAs against premature cleavage and polyadenylation within introns-a mechanism known as telescripting in metazoans-while also influencing a"],"journal":["Nature plants"],"pubmed_title":["The Arabidopsis U1 snRNP regulates mRNA 3'-end processing."],"pmcid":["PMC11489095"],"funding_grant_id":["LA2633-4/2","400681449/GRK2498 TP13"],"pubmed_authors":["Nagel L","Schmidtke C","Weber J","Rot G","Heilmann P","Schmutzer T","Mjema EY","Renneberg M","Laubinger S","Herold A","Droste-Borel I","Adler M","Mangilet AF","Schuler S","Streicher S","Macek B"],"additional_accession":[]},"is_claimable":false,"name":"The Arabidopsis U1 snRNP regulates mRNA 3'-end processing.","description":"The removal of introns by the spliceosome is a key gene regulatory mechanism in eukaryotes, with the U1 snRNP subunit playing a crucial role in the early stages of splicing. Studies in metazoans show that the U1 snRNP also conducts splicing-independent functions, but the lack of genetic tools and knowledge about U1 snRNP-associated proteins have limited the study of such splicing-independent functions in plants. Here we describe an RNA-centric approach that identified more than 200 proteins associated with the Arabidopsis U1 snRNP and revealed a tight link to mRNA cleavage and polyadenylation factors. Interestingly, we found that the U1 snRNP protects mRNAs against premature cleavage and polyadenylation within introns-a mechanism known as telescripting in metazoans-while also influencing a","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Oct","modification":"2025-04-04T01:11:31.259Z","creation":"2025-04-04T01:11:31.259Z"},"accession":"S-EPMC11489095","cross_references":{"pubmed":["39313562"],"doi":["10.1038/s41477-024-01796-8"]}}