biostudies-literatureUnknown7(1)Zhang JMetabolic memory (MM) is a major factor in the delayed wound healing observed in diabetic patients. While "Nocardia rubrum cell wall skeleton" (Nr-CWS) is utilized to enhance macrophage proliferation in immune diseases, its impact on MM wounds in diabetes is unclear. This study demonstrates that transient hyperglycemia leads to prolonged damage in vascular endothelial cells by decreasing METTL3 expression, leading to decreased RNA methylation and impaired cellular metabolism. Remarkably, Nr-CWS application increases METTL3 levels in these cells, facilitating the recovery of cell function. Further in vivo and in vitro analyses demonstrate that transient hyperglycemia-induced reduction in METTL3 hinders RNA methylation of the downstream gene Cds2, impacting mitochondrial function and energy Communications biology1348https://www.ebi.ac.uk/biostudies/studies/S-EPMC11489679biostudies-literatureNr-CWS regulates METTL3-mediated m⁶A modification of CDS2 mRNA in vascular endothelial cells and has prognostic significance.PMC11489679Ning QZhang JFan JChen FLi QWei WWang HJin PWang JZhu DZhang AfalseNr-CWS regulates METTL3-mediated m⁶A modification of CDS2 mRNA in vascular endothelial cells and has prognostic significance.Metabolic memory (MM) is a major factor in the delayed wound healing observed in diabetic patients. While "Nocardia rubrum cell wall skeleton" (Nr-CWS) is utilized to enhance macrophage proliferation in immune diseases, its impact on MM wounds in diabetes is unclear. This study demonstrates that transient hyperglycemia leads to prolonged damage in vascular endothelial cells by decreasing METTL3 expression, leading to decreased RNA methylation and impaired cellular metabolism. Remarkably, Nr-CWS application increases METTL3 levels in these cells, facilitating the recovery of cell function. Further in vivo and in vitro analyses demonstrate that transient hyperglycemia-induced reduction in METTL3 hinders RNA methylation of the downstream gene Cds2, impacting mitochondrial function and energy 2024-01-01T00:00:00Z2024 Oct2026-06-02T22:23:30.635Z2025-04-06T14:26:15.163ZS-EPMC114896793942463410.1038/s42003-024-07047-y