biostudies-literatureHu SHainan Province Science and Technology Special FundHainan Province Clinical Medical CenterCultivation Project of Provincial Clinical Medical Centre of Hainan Women and Children’s Medical CenterProject Subjects of 1126 Plan of Hainan Women and Children’s Medical CenterJoint Program on Health Science & Technology Innovation of Hainan ProvinceCultivation Project of Provincial Clinical Medical Centre of Hainan Women and Children's Medical Center24527https://www.ebi.ac.uk/biostudies/studies/S-EPMC11489682biostudies-literatureUnknown14(1)Urethral reconstruction presents a challenging issue in urology, primarily due to the limited availability of alternative materials for repair. The advancement of bioengineering technology has brought new hope to researchers, with a focus on the selection of appropriate biological scaffolds and seed cells. In order to find an ideal alternative material, we used platelet-rich fibrin as the bioscaffold and urothelial cells as the seed cells, meanwhile, we intended to investigate the effect of platelet-rich fibrin on the biological properties of urothelial cells. We transformed and characterised induced pluripotent stem cells into urothelial cells and prepared platelet-rich fibrin. Platelet-rich fibrin was cultured in a complex with urothelial cells to observe the effect of platelet-rich fibrScientific reportsThe effect of platelet-rich fibrin on the biological properties of urothelial cells.PMC11489682WSJK2024QN011QWYH202175ZDYF2024SHFZ105202105202301Hu SBu WLu YWan ZChen SZhao ZHan TfalseThe effect of platelet-rich fibrin on the biological properties of urothelial cells.Urethral reconstruction presents a challenging issue in urology, primarily due to the limited availability of alternative materials for repair. The advancement of bioengineering technology has brought new hope to researchers, with a focus on the selection of appropriate biological scaffolds and seed cells. In order to find an ideal alternative material, we used platelet-rich fibrin as the bioscaffold and urothelial cells as the seed cells, meanwhile, we intended to investigate the effect of platelet-rich fibrin on the biological properties of urothelial cells. We transformed and characterised induced pluripotent stem cells into urothelial cells and prepared platelet-rich fibrin. Platelet-rich fibrin was cultured in a complex with urothelial cells to observe the effect of platelet-rich fibr2024-01-01T00:00:00Z2024 Oct2025-04-04T02:20:52.305Z2025-04-04T02:20:52.305ZS-EPMC114896823942488110.1038/s41598-024-75699-1