biostudies-literatureUnknown6(11)Claria J<h4>Background & aims</h4>Patients with acutely decompensated (AD) cirrhosis are immunocompromised and particularly susceptible to infections. This study investigated the immunomodulatory actions of albumin by which this protein may lower the incidence of infections.<h4>Methods</h4>Blood immunophenotyping was performed in 11 patients with AD cirrhosis and 10 healthy volunteers (HV). Bulk and single-cell RNA sequencing (scRNA-seq) and flow cytometry were performed in peripheral blood mononuclear cells (PBMCs) from 20 patients with AD cirrhosis and 34 HV exposed to albumin. Albumin's effects on degranulation, phagocytosis, chemotaxis, and swarming of neutrophils from six patients with AD cirrhosis and nine HV were assessed by measuring myeloperoxidase enzymatic activity, the engulfment of flJHEP reports : innovation in hepatology101184https://www.ebi.ac.uk/biostudies/studies/S-EPMC11490805biostudies-literatureAlbumin reprograms the B cell transcriptional landscape and improves neutrophil antimicrobial function in patients with decompensated cirrhosis.PMC11490805Marcos-Fa XLopez-Vicario CAngeli PLozano JJTrebicka JMoreau RMarchese DArroyo VRomero-Grimaldo BWeiss EHernandez IAransay AMJimenez-Gracia LAguilar FVlagea ABernardi MHopke ACerutti AFernandez JJalan RMuldur SNieto JCClaria JIrimia DCasulleras MMagri GGiarracco EHeyn HfalseAlbumin reprograms the B cell transcriptional landscape and improves neutrophil antimicrobial function in patients with decompensated cirrhosis.<h4>Background & aims</h4>Patients with acutely decompensated (AD) cirrhosis are immunocompromised and particularly susceptible to infections. This study investigated the immunomodulatory actions of albumin by which this protein may lower the incidence of infections.<h4>Methods</h4>Blood immunophenotyping was performed in 11 patients with AD cirrhosis and 10 healthy volunteers (HV). Bulk and single-cell RNA sequencing (scRNA-seq) and flow cytometry were performed in peripheral blood mononuclear cells (PBMCs) from 20 patients with AD cirrhosis and 34 HV exposed to albumin. Albumin's effects on degranulation, phagocytosis, chemotaxis, and swarming of neutrophils from six patients with AD cirrhosis and nine HV were assessed by measuring myeloperoxidase enzymatic activity, the engulfment of fl2024-01-01T00:00:00Z2024 Nov2025-04-18T13:08:05.018Z2025-04-06T22:40:08.353ZS-EPMC114908053943489110.1016/j.jhepr.2024.101184