{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Gao Y"],"funding":["New Cornerstone Investigator Program","Xiangfu Lab Research Project","Fundamental Research Project","National Natural Science Foundation of China","Science and Technology Innovation Action Plan","National Key Research and Development Program of China"],"pagination":["nwae307"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11493095"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["11(11)"],"pubmed_abstract":["Abnormal levels of zinc ions within endo-lysosomes have been implicated in the progression of Alzheimer's disease (AD), yet the detection of low-concentration zinc ions at the organelle level remains challenging. Here we report the design of an endo-lysosome-targeted fluorescent reporter, Znluor<sub>ly</sub>, for imaging endogenous zinc ions. Znluor<sub>ly</sub> is constructed from an amphiphilic DNA framework (DNF) with programmable size and shape, which can encapsulate zinc-responsive fluorophores within its hydrophobic nanocavity. We find that the tetrahedral DNFs of 20 bp in the edge length are effectively located within endo-lysosomes, which can detect zinc ions with a detection limit of ∼31.9 nM (a sensitivity that is ∼2.5 times that of the free fluorophore). Given the organelle-targ"],"journal":["National science review"],"pubmed_title":["Targeted imaging of lysosomal zinc ions with a tetrahedral DNA framework fluorescent reporter."],"pmcid":["PMC11493095"],"funding_grant_id":["22325406","T2188102","12305400","XF012022E0100","22JC1401203","21991134","2023YFC3404200"],"pubmed_authors":["Geng S","Li J","Yan Q","Guo Z","Zhang J","Zhu Y","Li Q","Wang L","Liu X","Li W","Guan Y","Jia S","He W","Gao Y","Chen Y","Fan C","Zhu S"],"additional_accession":[]},"is_claimable":false,"name":"Targeted imaging of lysosomal zinc ions with a tetrahedral DNA framework fluorescent reporter.","description":"Abnormal levels of zinc ions within endo-lysosomes have been implicated in the progression of Alzheimer's disease (AD), yet the detection of low-concentration zinc ions at the organelle level remains challenging. Here we report the design of an endo-lysosome-targeted fluorescent reporter, Znluor<sub>ly</sub>, for imaging endogenous zinc ions. Znluor<sub>ly</sub> is constructed from an amphiphilic DNA framework (DNF) with programmable size and shape, which can encapsulate zinc-responsive fluorophores within its hydrophobic nanocavity. We find that the tetrahedral DNFs of 20 bp in the edge length are effectively located within endo-lysosomes, which can detect zinc ions with a detection limit of ∼31.9 nM (a sensitivity that is ∼2.5 times that of the free fluorophore). Given the organelle-targ","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Nov","modification":"2025-04-27T02:10:13.186Z","creation":"2025-04-06T18:29:07.088Z"},"accession":"S-EPMC11493095","cross_references":{"pubmed":["39440260"],"doi":["10.1093/nsr/nwae307"]}}