biostudies-literatureMa XGuizhou Institute of Technology High-level Talent Scientific Research Start-up FundNational Outstanding Youth Science Fund Project of National Natural Science Foundation of ChinaZunyi Technology and Big data Bureau Moutai institute Joint Science and Technology Research and Development Project24962https://www.ebi.ac.uk/biostudies/studies/S-EPMC11496540biostudies-literatureUnknown14(1)To determine the synergistic effect and mechanism of AO/854, a new Bloom syndrome protein (BLM) helicase inhibitor, and cisplatin (CDDP), a DNA-crosslinking agent, cell viability assays, neutral comet assays, and Western blotting (WB) were performed on prostate cancer (PCa) cells. According to our findings, combining AO/854 and CDDP enhanced the antiproliferative capabilities of PC3 cell lines. As evidenced by the upregulation of γH2AX, cleaved caspase-3/caspase-3, and BAX/Bcl-2, AO/854 dramatically increased PC3 apoptosis and DNA damage induced by CDDP. Furthermore, combining AO/854 and CDDP synergistically inhibited PC3 cell migration and invasion. In addition, AO/854 inhibited CDDP-induced S-phase cell-cycle arrest in PC3 cells while enhancing G2/M-phase cell-cycle arrest. In vivo, the Scientific reportsSynergistic effects of bloom helicase (BLM) inhibitor AO/854 with cisplatin in prostate cancer.PMC11496540318602422023GCC066ZunaShiJiaoHe HZ zi[2020]316Tian FXiao YMa XHuang MSong DChen XXu HfalseSynergistic effects of bloom helicase (BLM) inhibitor AO/854 with cisplatin in prostate cancer.To determine the synergistic effect and mechanism of AO/854, a new Bloom syndrome protein (BLM) helicase inhibitor, and cisplatin (CDDP), a DNA-crosslinking agent, cell viability assays, neutral comet assays, and Western blotting (WB) were performed on prostate cancer (PCa) cells. According to our findings, combining AO/854 and CDDP enhanced the antiproliferative capabilities of PC3 cell lines. As evidenced by the upregulation of γH2AX, cleaved caspase-3/caspase-3, and BAX/Bcl-2, AO/854 dramatically increased PC3 apoptosis and DNA damage induced by CDDP. Furthermore, combining AO/854 and CDDP synergistically inhibited PC3 cell migration and invasion. In addition, AO/854 inhibited CDDP-induced S-phase cell-cycle arrest in PC3 cells while enhancing G2/M-phase cell-cycle arrest. In vivo, the 2024-01-01T00:00:00Z2024 Oct2026-06-02T21:56:39.32Z2025-04-04T02:57:38.822ZS-EPMC114965403943853710.1038/s41598-024-75938-5