biostudies-literatureSong XNational Natural Science Foundation of ChinaShanghai Municipal Key Clinical SpecialtyNational Key Research and Development Program of ChinaBiomaterials and Regenerative Medicine Institute Cooperative Research Project of Shanghai Jiao Tong University School of Medicinee2405420https://www.ebi.ac.uk/biostudies/studies/S-EPMC11497002biostudies-literatureUnknown11(39)Functional segmental trachea reconstruction is a critical concern in thoracic surgery, and tissue-engineered trachea (TET) holds promise as a potential solution. However, current TET falls short in fully restoring physiological function due to the lack of the intricate multi-tissue structure found in natural trachea. In this research, a multi-tissue integrated tissue-engineered trachea (MI-TET) is successfully developed by orderly assembling various cells (chondrocytes, fibroblasts and epithelial cells) on 3D-printed PGS bioelastomer scaffolds. The MI-TET closely resembles the complex structures of natural trachea and achieves the integrated regeneration of four essential tracheal components: C-shaped cartilage ring, O-shaped vascularized fiber ring, axial fiber bundle, and airway epitheliAdvanced science (Weinheim, Baden-Wurttemberg, Germany)Multi-Tissue Integrated Tissue-Engineered Trachea Regeneration Based on 3D Printed Bioelastomer Scaffolds.PMC114970022023YFC241700082102211shslczdzk066012022LHA07Liu YWang QHuang JWang SZhang YQin XZhou GLei DSong XLi KLuo BZhang PfalseMulti-Tissue Integrated Tissue-Engineered Trachea Regeneration Based on 3D Printed Bioelastomer Scaffolds.Functional segmental trachea reconstruction is a critical concern in thoracic surgery, and tissue-engineered trachea (TET) holds promise as a potential solution. However, current TET falls short in fully restoring physiological function due to the lack of the intricate multi-tissue structure found in natural trachea. In this research, a multi-tissue integrated tissue-engineered trachea (MI-TET) is successfully developed by orderly assembling various cells (chondrocytes, fibroblasts and epithelial cells) on 3D-printed PGS bioelastomer scaffolds. The MI-TET closely resembles the complex structures of natural trachea and achieves the integrated regeneration of four essential tracheal components: C-shaped cartilage ring, O-shaped vascularized fiber ring, axial fiber bundle, and airway epitheli2024-01-01T00:00:00Z2024 Oct2025-04-19T13:39:32.573Z2025-04-19T13:39:32.573ZS-EPMC114970023915915610.1002/advs.202405420