biostudies-literatureWang ASNIDDK NIH HHSNational Institute of Diabetes and Digestive and Kidney DiseasesNational Institutes of Health122577https://www.ebi.ac.uk/biostudies/studies/S-EPMC11497585biostudies-literatureUnknown309The relationship between the mechanical forces associated with bowel movement and colonic mucosal physiology is understudied. This is partly due to the limited availability of physiologically relevant fecal models that can exert these mechanical stimuli in in vitro colon models in a simple-to-implement manner. In this report, we created a mucus-coated fecal surrogate that was magnetically propelled to produce a controllable sweeping mechanical stimulation on primary intestinal epithelial cell monolayers. The mucus layer was derived from purified porcine stomach mucins, which were first modified with reactive vinyl sulfone (VS) groups followed by reaction with a thiol crosslinker (PEG-4SH) via a Michael addition click reaction. Formation of mucus hydrogel network was achieved at the optimalBiomaterialsMucus-coated, magnetically-propelled fecal surrogate to mimic fecal shear forces on colonic epithelium.PMC11497585R43 DK121580DK120606DK121580R01 DK120606R44 DK121580Sims CEAllbritton NLWang YWang ASVillegas-Novoa CfalseMucus-coated, magnetically-propelled fecal surrogate to mimic fecal shear forces on colonic epithelium.The relationship between the mechanical forces associated with bowel movement and colonic mucosal physiology is understudied. This is partly due to the limited availability of physiologically relevant fecal models that can exert these mechanical stimuli in in vitro colon models in a simple-to-implement manner. In this report, we created a mucus-coated fecal surrogate that was magnetically propelled to produce a controllable sweeping mechanical stimulation on primary intestinal epithelial cell monolayers. The mucus layer was derived from purified porcine stomach mucins, which were first modified with reactive vinyl sulfone (VS) groups followed by reaction with a thiol crosslinker (PEG-4SH) via a Michael addition click reaction. Formation of mucus hydrogel network was achieved at the optimal2024-01-01T00:00:00Z2024 Sep2026-05-29T06:58:23.095Z2026-04-08T03:51:27.683ZS-EPMC114975853867722110.1016/j.biomaterials.2024.122577