biostudies-literatureUnknown13(2)Guo FBasal cell carcinoma (BCC) is a common type of skin cancer. Conventional approaches to BCC diagnosis often involve invasive histological examinations that can distort or even destroy information derived from the biomolecules in the sample. Therefore, a non-invasive, label-free examination method for the clinical diagnosis of BCC represents a critical advance. This study combined spectral- and time-resolved two-photon microscopy with a spectral phasor to extract rich biochemical information describing macroscopic tumor morphology and microscopic tumor metabolism. The proposed optical imaging technique achieved the rapid and efficient separation of tumor structures in systematic research conducted on normal and BCC human skin tissues. The results demonstrate that a combination of multidimensNanophotonics (Berlin, Germany)217-227https://www.ebi.ac.uk/biostudies/studies/S-EPMC11501964biostudies-literatureMultidimensional quantitative characterization of basal cell carcinoma by spectral- and time-resolved two-photon microscopy.PMC11501964Liu LHe JLu YQu JShen BLiao CLiu YLi YGuo JWang SLin FGuo FChen YWang YHu RfalseMultidimensional quantitative characterization of basal cell carcinoma by spectral- and time-resolved two-photon microscopy.Basal cell carcinoma (BCC) is a common type of skin cancer. Conventional approaches to BCC diagnosis often involve invasive histological examinations that can distort or even destroy information derived from the biomolecules in the sample. Therefore, a non-invasive, label-free examination method for the clinical diagnosis of BCC represents a critical advance. This study combined spectral- and time-resolved two-photon microscopy with a spectral phasor to extract rich biochemical information describing macroscopic tumor morphology and microscopic tumor metabolism. The proposed optical imaging technique achieved the rapid and efficient separation of tumor structures in systematic research conducted on normal and BCC human skin tissues. The results demonstrate that a combination of multidimens2024-01-01T00:00:00Z2024 Jan2025-04-18T12:54:39.177Z2025-04-06T22:17:52.824ZS-EPMC115019643963530210.1515/nanoph-2023-0722