{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Huang KY"],"funding":["Army ERDC 6.1 Basic Research program, DoD Tri-Service Biotechnology for a Resilient Supply Chain program","United States Department of Defense | United States Army | U.S. Army Research, Development and Engineering Command | Army Research Office","ERDC Basic Research program, DoD Tri-Service Biotechnology for a Resilient Supply Chain program","United States Department of Defense | United States Army | US Army Corps of Engineers | Engineer Research and Development Center","United States Department of Defense | United States Army | US Army Corps of Engineers | Engineer Research and Development Center (U.S. Army Engineer Research and Development Center)","United States Department of Defense | United States Army | U.S. Army Research, Development and Engineering Command | Army Research Office (ARO)"],"pagination":["9200"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11502933"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["15(1)"],"pubmed_abstract":["A sustainable operation for harvesting metals in the lanthanide series is needed to meet the rising demand for rare earth elements across diverse global industries. However, existing methods are limited in their capacity for detection and capture at environmentally and industrially relevant lanthanide concentrations. Supercharged fluorescent proteins have solvent-exposed, negatively charged residues that potentially create multiple direct chelation pockets for free lanthanide cations. Here, we demonstrate that negatively supercharged proteins can bind and quantitatively report concentrations of lanthanides via an underutilized lanthanide-to-chromophore pathway of energy transfer. The top-performing sensors detect lanthanides in the micromolar to millimolar range and remain unperturbed by e"],"journal":["Nature communications"],"pubmed_title":["Supercharged fluorescent proteins detect lanthanides via direct antennae signaling."],"pmcid":["PMC11502933"],"funding_grant_id":["W911NF2320089","W912HZ-22-2-0037","W911NF2220246","W912HZ-22-Q-0016"],"pubmed_authors":["Huang KY","Ellington AD","Cardenas L","Walker DJF"],"additional_accession":[]},"is_claimable":false,"name":"Supercharged fluorescent proteins detect lanthanides via direct antennae signaling.","description":"A sustainable operation for harvesting metals in the lanthanide series is needed to meet the rising demand for rare earth elements across diverse global industries. However, existing methods are limited in their capacity for detection and capture at environmentally and industrially relevant lanthanide concentrations. Supercharged fluorescent proteins have solvent-exposed, negatively charged residues that potentially create multiple direct chelation pockets for free lanthanide cations. Here, we demonstrate that negatively supercharged proteins can bind and quantitatively report concentrations of lanthanides via an underutilized lanthanide-to-chromophore pathway of energy transfer. The top-performing sensors detect lanthanides in the micromolar to millimolar range and remain unperturbed by e","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Oct","modification":"2026-06-03T01:00:36.799Z","creation":"2025-04-04T12:00:25.657Z"},"accession":"S-EPMC11502933","cross_references":{"pubmed":["39448572"],"doi":["10.1038/s41467-024-53106-7"]}}