biostudies-literatureCouto-Lima CAFundação de Amparo à Pesquisa do Estado de São Paulo - FAPESPConselho Nacional de Desenvolvimento Científico e Tecnológico-CNPq1258https://www.ebi.ac.uk/biostudies/studies/S-EPMC11506464biostudies-literatureUnknown14(10)EMC1 is part of the endoplasmic reticulum (ER) membrane protein complex, whose functions include the insertion of transmembrane proteins into the ER membrane, ER-mitochondria contact, and lipid exchange. Here, we show that the <i>Drosophila melanogaster EMC1</i> gene is expressed in the somatic musculature and the protein localizes to the sarcoplasmic reticulum (SR) network. Muscle-specific <i>EMC1</i> RNAi led to severe motility defects and partial late pupae/early adulthood lethality, phenotypes that are rescued by co-expression with an <i>EMC1</i> transgene. Motility impairment in EMC1-depleted flies was associated with aberrations in muscle morphology in embryos, larvae, and adults, including tortuous and misaligned fibers with reduced size and weakness. They were also associated with BiomoleculesEMC1 Is Required for the Sarcoplasmic Reticulum and Mitochondrial Functions in the &lt;i&gt;Drosophila&lt;/i&gt; Muscle.PMC115064642016/16896-92014/18189-5311347/2011-82022/05632-42013/21242-22015/13396-52021/06711-2506780/2013-92012/17890-62011/50962-82004/08868-02009/54014-72010/17259-92011/16666-22010/11812-8Alberici LCTrinca VAnhezini LOliveira MTEspreafico EMPeixoto PMRamos RGPMachado MCRMolina RADSMonesi NCouto-Lima CAda Silva RRLopes GSColon DFfalseEMC1 Is Required for the Sarcoplasmic Reticulum and Mitochondrial Functions in the &lt;i&gt;Drosophila&lt;/i&gt; Muscle.EMC1 is part of the endoplasmic reticulum (ER) membrane protein complex, whose functions include the insertion of transmembrane proteins into the ER membrane, ER-mitochondria contact, and lipid exchange. Here, we show that the <i>Drosophila melanogaster EMC1</i> gene is expressed in the somatic musculature and the protein localizes to the sarcoplasmic reticulum (SR) network. Muscle-specific <i>EMC1</i> RNAi led to severe motility defects and partial late pupae/early adulthood lethality, phenotypes that are rescued by co-expression with an <i>EMC1</i> transgene. Motility impairment in EMC1-depleted flies was associated with aberrations in muscle morphology in embryos, larvae, and adults, including tortuous and misaligned fibers with reduced size and weakness. They were also associated with 2024-01-01T00:00:00Z2024 Oct2025-04-03T23:51:28.465Z2025-04-03T23:51:28.465ZS-EPMC115064643945619110.3390/biom14101258