biostudies-literatureTorres-Sanchez ESistema General de Regalías ColombiaSistema General de Regalías (SGR)3275https://www.ebi.ac.uk/biostudies/studies/S-EPMC11506952biostudies-literatureUnknown13(20)Sacha Inchi (<i>Plukenetia volubilis</i>) oil press-cake (SIPC) represents a new source of proteins of high biological value, with promissory food applications. However, knowledge of these proteins remains limited. In this study, a Sacha Inchi protein concentrate (SPC) was extracted from the SIPC, and proteomic analysis was performed to identify the major alkaline-soluble proteins. The electrophoretic profile highlighted the efficacy of alkaline pH and moderate temperature to extract the major proteins, from which a group of proteins, not previously reported, were registered. LC-MS/MS analyses produced abundant high-quality fragmentation spectra. Utilizing the Euphorbiaceae database (DB), 226 proteins were identified, with numerous well-assigned spectra remaining unidentified. PEAKS StudioFoods (Basel, Switzerland)Proteomic Analysis of the Major Alkali-Soluble Inca Peanut (<i>Plukenetia volubilis</i>) Proteins.PMC115069522020000100169BPIN2020000100169Morato EGutierrez LFTorres-Sanchez EHernandez-Ledesma BfalseProteomic Analysis of the Major Alkali-Soluble Inca Peanut (<i>Plukenetia volubilis</i>) Proteins.Sacha Inchi (<i>Plukenetia volubilis</i>) oil press-cake (SIPC) represents a new source of proteins of high biological value, with promissory food applications. However, knowledge of these proteins remains limited. In this study, a Sacha Inchi protein concentrate (SPC) was extracted from the SIPC, and proteomic analysis was performed to identify the major alkaline-soluble proteins. The electrophoretic profile highlighted the efficacy of alkaline pH and moderate temperature to extract the major proteins, from which a group of proteins, not previously reported, were registered. LC-MS/MS analyses produced abundant high-quality fragmentation spectra. Utilizing the Euphorbiaceae database (DB), 226 proteins were identified, with numerous well-assigned spectra remaining unidentified. PEAKS Studio2024-01-01T00:00:00Z2024 Oct2026-07-08T03:18:39.276Z2025-04-04T11:42:51.446ZS-EPMC115069523945633710.3390/foods13203275