biostudies-literatureUnknownTiwari MNIAID NIH HHSNIGMS NIH HHSCellular adaptations to change often involve post-translational modifications of nuclear and cytoplasmic proteins. An example found in protists and plants is the modification of serine and threonine residues of dozens to hundreds of nucleocytoplasmic proteins with a single fucose (O-Fuc). A nucleocytoplasmic O-fucosyltransferase (OFT) occurs in the pathogen <i>Toxoplasma gondii</i>, the social amoeba <i>Dictyostelium</i>, and higher plants, where it is called Spy because mutants have a spindly appearance. O-fucosylation, which is required for optimal proliferation of <i>Toxoplasma</i> and <i>Dictyostelium</i>, is paralogous to the O-GlcNAcylation of nucleocytoplasmic proteins of plants and animals that is involved in stress and nutritional responses. O-Fuc was first discovered in <i>ToxoplbioRxiv : the preprint server for biology2024.10.15.618526https://www.ebi.ac.uk/biostudies/studies/S-EPMC11507795biostudies-literatureNovel antibodies detect nucleocytoplasmic O-fucose in protist pathogens, cellular slime molds, and plants.PMC11507795R01 GM129324R35 GM148433T32 AI060546Samuelson JOlszewski NGoyal MHaltiwanger RSMatsumoto KWest CMGas-Pascual EGrafe MOrlando RTiwari MGraf RPopov MfalseNovel antibodies detect nucleocytoplasmic O-fucose in protist pathogens, cellular slime molds, and plants.Cellular adaptations to change often involve post-translational modifications of nuclear and cytoplasmic proteins. An example found in protists and plants is the modification of serine and threonine residues of dozens to hundreds of nucleocytoplasmic proteins with a single fucose (O-Fuc). A nucleocytoplasmic O-fucosyltransferase (OFT) occurs in the pathogen <i>Toxoplasma gondii</i>, the social amoeba <i>Dictyostelium</i>, and higher plants, where it is called Spy because mutants have a spindly appearance. O-fucosylation, which is required for optimal proliferation of <i>Toxoplasma</i> and <i>Dictyostelium</i>, is paralogous to the O-GlcNAcylation of nucleocytoplasmic proteins of plants and animals that is involved in stress and nutritional responses. O-Fuc was first discovered in <i>Toxopl2024-01-01T00:00:00Z2024 Oct2026-07-01T03:17:45.719Z2025-04-06T01:57:36.98ZS-EPMC115077953946406510.1101/2024.10.15.618526