{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["19(10)"],"submitter":["Leon C"],"pubmed_abstract":["<h4>Background</h4>The aim of our study was to test different anesthetic mixtures in order to identify the most suitable one for a surgical cardiac ischemia-reperfusion model in mice.<h4>Methods</h4>1) Sixty four mice were submitted to one of the 6 combinations of ketamine or alfaxalone associated to xylazine, medetomidine or midazolam. Depth and quality of anesthesia were evaluated via 5 reflex scores. 2) Impact of analgesic (buprenorphine or butorphanol), anesthesia reversal (with atipamezole) and surgery (cardiac ischemia-reperfusion surgery) have been tested in the selected protocols. 3) infarction size has been measured with TTC (Triphenyl Tetrazolium Chloride) method in mice anesthetized with best protocols.<h4>Results</h4>Protocol involving medetomidine induced the longest surgical "],"journal":["PloS one"],"pagination":["e0309799"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11508169"],"repository":["biostudies-literature"],"pubmed_title":["Evaluation of general anesthesia protocols for a highly controlled cardiac ischemia-reperfusion model in mice."],"pmcid":["PMC11508169"],"pubmed_authors":["Bidaux G","Gomez L","Ovize M","Ruelle A","Vogt C","Leon C","Augeul L","Chiari P","Geoffray J","Pillot B"],"additional_accession":[]},"is_claimable":false,"name":"Evaluation of general anesthesia protocols for a highly controlled cardiac ischemia-reperfusion model in mice.","description":"<h4>Background</h4>The aim of our study was to test different anesthetic mixtures in order to identify the most suitable one for a surgical cardiac ischemia-reperfusion model in mice.<h4>Methods</h4>1) Sixty four mice were submitted to one of the 6 combinations of ketamine or alfaxalone associated to xylazine, medetomidine or midazolam. Depth and quality of anesthesia were evaluated via 5 reflex scores. 2) Impact of analgesic (buprenorphine or butorphanol), anesthesia reversal (with atipamezole) and surgery (cardiac ischemia-reperfusion surgery) have been tested in the selected protocols. 3) infarction size has been measured with TTC (Triphenyl Tetrazolium Chloride) method in mice anesthetized with best protocols.<h4>Results</h4>Protocol involving medetomidine induced the longest surgical ","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024","modification":"2025-04-03T23:52:35.617Z","creation":"2025-04-03T23:52:35.617Z"},"accession":"S-EPMC11508169","cross_references":{"pubmed":["39453969"],"doi":["10.1371/journal.pone.0309799"]}}