biostudies-literatureUnknown19(10)Leon C<h4>Background</h4>The aim of our study was to test different anesthetic mixtures in order to identify the most suitable one for a surgical cardiac ischemia-reperfusion model in mice.<h4>Methods</h4>1) Sixty four mice were submitted to one of the 6 combinations of ketamine or alfaxalone associated to xylazine, medetomidine or midazolam. Depth and quality of anesthesia were evaluated via 5 reflex scores. 2) Impact of analgesic (buprenorphine or butorphanol), anesthesia reversal (with atipamezole) and surgery (cardiac ischemia-reperfusion surgery) have been tested in the selected protocols. 3) infarction size has been measured with TTC (Triphenyl Tetrazolium Chloride) method in mice anesthetized with best protocols.<h4>Results</h4>Protocol involving medetomidine induced the longest surgical PloS onee0309799https://www.ebi.ac.uk/biostudies/studies/S-EPMC11508169biostudies-literatureEvaluation of general anesthesia protocols for a highly controlled cardiac ischemia-reperfusion model in mice.PMC11508169Bidaux GGomez LOvize MRuelle AVogt CLeon CAugeul LChiari PGeoffray JPillot BfalseEvaluation of general anesthesia protocols for a highly controlled cardiac ischemia-reperfusion model in mice.<h4>Background</h4>The aim of our study was to test different anesthetic mixtures in order to identify the most suitable one for a surgical cardiac ischemia-reperfusion model in mice.<h4>Methods</h4>1) Sixty four mice were submitted to one of the 6 combinations of ketamine or alfaxalone associated to xylazine, medetomidine or midazolam. Depth and quality of anesthesia were evaluated via 5 reflex scores. 2) Impact of analgesic (buprenorphine or butorphanol), anesthesia reversal (with atipamezole) and surgery (cardiac ischemia-reperfusion surgery) have been tested in the selected protocols. 3) infarction size has been measured with TTC (Triphenyl Tetrazolium Chloride) method in mice anesthetized with best protocols.<h4>Results</h4>Protocol involving medetomidine induced the longest surgical 2024-01-01T00:00:00Z20242025-04-03T23:52:35.617Z2025-04-03T23:52:35.617ZS-EPMC115081693945396910.1371/journal.pone.0309799