biostudies-literatureBuliga DIUnitatea Executiva Pentru Finantarea Invatamantului Superior a Cercetarii Dezvoltarii si InovariiNational University of Science and Technology POLITEHNICA Bucharest434https://www.ebi.ac.uk/biostudies/studies/S-EPMC11509733biostudies-literatureUnknown22(10)Phycocyanin was extracted from <i>Spirulina platensis</i> using conventional extraction (CE), direct ultrasonic-assisted extraction (direct UAE), indirect ultrasonic-assisted extraction (indirect UAE), and microwave-assisted extraction (MAE) methods at different temperatures, extraction intervals, stirring rate, and power intensities while maintaining the same algae to solvent ratio (1:15 <i>w/v</i>). The optimization of the extraction parameters indicated that the direct UAE yielded the highest phycocyanin concentration (29.31 ± 0.33 mg/mL) and antioxidant activity (23.6 ± 0.56 mg TE/g algae), while MAE achieved the highest purity (Rp = 0.5 ± 0.002). Based on the R_P value, phycocyanin extract obtained by MAE (1:15 <i>w/v</i> algae to solvent ratio, 40 min, 40 °C, and 900 rpm) wMarine drugsPhycocyanin-Loaded Alginate-Based Hydrogel Synthesis and Characterization.PMC11509733PN-III-P2-2.1-PED-2021-ctr.no. 672PED/2022PubArt ProgramPN 2307/29.12.2022 within PNCDI IVMocanu ARusen EBrincoveanu ODiacon ABoscornea ACBuliga DIToader GCalinescu IfalsePhycocyanin-Loaded Alginate-Based Hydrogel Synthesis and Characterization.Phycocyanin was extracted from <i>Spirulina platensis</i> using conventional extraction (CE), direct ultrasonic-assisted extraction (direct UAE), indirect ultrasonic-assisted extraction (indirect UAE), and microwave-assisted extraction (MAE) methods at different temperatures, extraction intervals, stirring rate, and power intensities while maintaining the same algae to solvent ratio (1:15 <i>w/v</i>). The optimization of the extraction parameters indicated that the direct UAE yielded the highest phycocyanin concentration (29.31 ± 0.33 mg/mL) and antioxidant activity (23.6 ± 0.56 mg TE/g algae), while MAE achieved the highest purity (Rp = 0.5 ± 0.002). Based on the R_P value, phycocyanin extract obtained by MAE (1:15 <i>w/v</i> algae to solvent ratio, 40 min, 40 °C, and 900 rpm) w2024-01-01T00:00:00Z2024 Sep2025-04-26T00:18:40.963Z2025-04-06T09:40:49.817ZS-EPMC115097333945284210.3390/md22100434