biostudies-literatureGe JNatural Science Foundation of Henan Province;Science and Technology Research and Development Plan Joint Fund Project of Henan Province;Scientific and Technological Projects Entrusted by Enterprises2093https://www.ebi.ac.uk/biostudies/studies/S-EPMC11509934biostudies-literatureUnknown12(10)Fusarium head blight in wheat is mainly caused by <i>Fusarium graminearum</i> and results in significant economic losses. Coenzyme Q (CoQ) is ubiquitously produced across organisms and functions as a hydrogen carrier in energy metabolism. While UbiH in <i>Escherichia coli</i> serves as a hydroxylase in CoQ biosynthesis, its role in phytopathogenic fungi is not well understood. This study explored the role of the hydroxylase FgUbiH in <i>F. graminearum</i>. Using a <i>FgUbiH</i> deletion mutant, we observed reduced hyphal growth, conidial production, germination, toxin synthesis, and pathogenicity compared to the wild-type. A transcriptome analysis indicated <i>FgUbiH</i>'s involvement in regulating carbohydrate and amino acid metabolism. Deletion of <i>FgUbiH</i> impaired mitochondrial funMicroorganismsFgUbiH Is Essential for Vegetative Development, Energy Metabolism, and Antioxidant Activity in &lt;i&gt;Fusarium graminearum&lt;/i&gt;.PMC11509934242300421106; 222103810063; H2022sw175Ma PTang LHu YZhai HLv YWei SLei YGe JZhao FZhang SZhou YWu XfalseFgUbiH Is Essential for Vegetative Development, Energy Metabolism, and Antioxidant Activity in &lt;i&gt;Fusarium graminearum&lt;/i&gt;.Fusarium head blight in wheat is mainly caused by <i>Fusarium graminearum</i> and results in significant economic losses. Coenzyme Q (CoQ) is ubiquitously produced across organisms and functions as a hydrogen carrier in energy metabolism. While UbiH in <i>Escherichia coli</i> serves as a hydroxylase in CoQ biosynthesis, its role in phytopathogenic fungi is not well understood. This study explored the role of the hydroxylase FgUbiH in <i>F. graminearum</i>. Using a <i>FgUbiH</i> deletion mutant, we observed reduced hyphal growth, conidial production, germination, toxin synthesis, and pathogenicity compared to the wild-type. A transcriptome analysis indicated <i>FgUbiH</i>'s involvement in regulating carbohydrate and amino acid metabolism. Deletion of <i>FgUbiH</i> impaired mitochondrial fun2024-01-01T00:00:00Z2024 Oct2025-04-19T13:39:26.047Z2025-04-19T13:39:26.047ZS-EPMC115099343945840310.3390/microorganisms12102093