biostudies-literatureUnknown12(10)Arnold MJObligate anaerobic beer spoilage bacteria have been a menace to the brewing industry for several decades. Technological advances in the brewing process aimed at suppressing aerobic spoilers gave rise to problems with obligate anaerobes. In previous studies, the metabolic spectrum of <i>Pectinatus</i> and <i>Megasphaera</i> species has been described, but their metabolism in the beer environment remains largely unknown. We used high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GCMS) to further characterize beer spoiled by 30 different strains from six beer-spoiling species of <i>Pectinatus</i> and <i>Megasphaera</i> (<i>P. cerevisiiphilus</i>, <i>P. frisingensiMicroorganisms2045https://www.ebi.ac.uk/biostudies/studies/S-EPMC11510336biostudies-literatureCarbohydrate Metabolism Differentiates <i>Pectinatus</i> and <i>Megasphaera</i> Species Growing in Beer.PMC11510336Kurniawan YNSuzuki KLiebl WEhrmann MAArnold MJRitter SWBecker TMfalseCarbohydrate Metabolism Differentiates <i>Pectinatus</i> and <i>Megasphaera</i> Species Growing in Beer.Obligate anaerobic beer spoilage bacteria have been a menace to the brewing industry for several decades. Technological advances in the brewing process aimed at suppressing aerobic spoilers gave rise to problems with obligate anaerobes. In previous studies, the metabolic spectrum of <i>Pectinatus</i> and <i>Megasphaera</i> species has been described, but their metabolism in the beer environment remains largely unknown. We used high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GCMS) to further characterize beer spoiled by 30 different strains from six beer-spoiling species of <i>Pectinatus</i> and <i>Megasphaera</i> (<i>P. cerevisiiphilus</i>, <i>P. frisingensi2024-01-01T00:00:00Z2024 Oct2026-04-08T19:47:21.559Z2025-04-06T18:27:32.39ZS-EPMC115103363945835510.3390/microorganisms12102045