{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Moran JC"],"funding":["NICHD NIH HHS","NIGMS NIH HHS"],"pagination":["eadm9238"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11510358"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["385(6706)"],"pubmed_abstract":["The human mitochondrial genome encodes crucial oxidative phosphorylation system proteins, pivotal for aerobic energy transduction. They are translated from nine monocistronic and two bicistronic transcripts whose native structures remain unexplored, posing a gap in understanding mitochondrial gene expression. In this work, we devised the mitochondrial dimethyl sulfate mutational profiling with sequencing (mitoDMS-MaPseq) method and applied detection of RNA folding ensembles using expectation-maximization (DREEM) clustering to unravel the native mitochondrial messenger RNA (mt-mRNA) structurome in wild-type (WT) and leucine-rich pentatricopeptide repeat-containing protein (LRPPRC)-deficient cells. Our findings elucidate LRPPRC's role as a holdase contributing to maintaining mt-mRNA folding "],"journal":["Science (New York, N.Y.)"],"pubmed_title":["The human mitochondrial mRNA structurome reveals mechanisms of gene expression."],"pmcid":["PMC11510358"],"funding_grant_id":["F30 HD107939","R35 GM118141"],"pubmed_authors":["Rouskin S","Fontanesi F","Brivanlou A","Barrientos A","Moran JC","Brischigliaro M"],"additional_accession":[]},"is_claimable":false,"name":"The human mitochondrial mRNA structurome reveals mechanisms of gene expression.","description":"The human mitochondrial genome encodes crucial oxidative phosphorylation system proteins, pivotal for aerobic energy transduction. They are translated from nine monocistronic and two bicistronic transcripts whose native structures remain unexplored, posing a gap in understanding mitochondrial gene expression. In this work, we devised the mitochondrial dimethyl sulfate mutational profiling with sequencing (mitoDMS-MaPseq) method and applied detection of RNA folding ensembles using expectation-maximization (DREEM) clustering to unravel the native mitochondrial messenger RNA (mt-mRNA) structurome in wild-type (WT) and leucine-rich pentatricopeptide repeat-containing protein (LRPPRC)-deficient cells. Our findings elucidate LRPPRC's role as a holdase contributing to maintaining mt-mRNA folding ","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Jul","modification":"2026-03-16T08:19:05.89Z","creation":"2025-08-15T03:06:08.221Z"},"accession":"S-EPMC11510358","cross_references":{"pubmed":["39024447"],"doi":["10.1126/science.adm9238"]}}