biostudies-literatureFlores DFranceAgriMer82https://www.ebi.ac.uk/biostudies/studies/S-EPMC11510656biostudies-literatureUnknown7(5)Polyphenols are responsible for wine colour and astringency, and, as antioxidants, they also have beneficial health properties. In this work, we developed a robust full-scan high-resolution mass spectrometry method for the quantification of 90 phenolic compounds in wine samples (either red, rosé, or white wine), using a UHPLC-Orbitrap^TM system. With this method, we could conduct a detailed analysis of phenolic compounds in red, rosé, and white wines with great selectivity due to sub-ppm mass accuracy. Moreover, accessing the full-scan spectrum enabled us to monitor all the other compounds detected in the sample, facilitating the adaptability of this method to new phenolic compounds if needed.Methods and protocolsA Versatile Ultra-High-Performance Liquid Chromatography-Full-Scan High-Resolution Mass Spectrometry Method to Quantify Wine Polyphenols.PMC11510656CASDAR n° CNEDP0922005233 "ODeVi: Oenotypage pour développer les variétés de vigne du futur, adaptées au changement climatique"Sommerer NDias ALSFlores DMeudec EfalseA Versatile Ultra-High-Performance Liquid Chromatography-Full-Scan High-Resolution Mass Spectrometry Method to Quantify Wine Polyphenols.Polyphenols are responsible for wine colour and astringency, and, as antioxidants, they also have beneficial health properties. In this work, we developed a robust full-scan high-resolution mass spectrometry method for the quantification of 90 phenolic compounds in wine samples (either red, rosé, or white wine), using a UHPLC-Orbitrap^TM system. With this method, we could conduct a detailed analysis of phenolic compounds in red, rosé, and white wines with great selectivity due to sub-ppm mass accuracy. Moreover, accessing the full-scan spectrum enabled us to monitor all the other compounds detected in the sample, facilitating the adaptability of this method to new phenolic compounds if needed.2024-01-01T00:00:00Z2024 Oct2025-04-04T02:22:17.599Z2025-04-04T02:22:17.599ZS-EPMC115106563945279610.3390/mps7050082